Structure of the Mucosal and Stool Microbiome in Lynch Syndrome
- PMID: 32240601
- PMCID: PMC7453618
- DOI: 10.1016/j.chom.2020.03.005
Structure of the Mucosal and Stool Microbiome in Lynch Syndrome
Abstract
The gut microbiota has been associated with colorectal cancer (CRC), but causal alterations preceding CRC have not been elucidated. To prospectively assess microbiome changes prior to colorectal neoplasia, we investigated samples from 100 Lynch syndrome patients using 16S rRNA gene sequencing of colon biopsies, coupled with metagenomic and metatranscriptomic sequencing of feces. Colectomy and CRC history represented the largest effects on microbiome profiles. A subset of Clostridiaceae were depleted in stool corresponding with baseline adenomas, while Desulfovibrio was enriched both in stool and in mucosal biopsies. A classifier leveraging stool metatranscriptomes resulted in modest power to predict interval development of preneoplastic colonic adenoma. Predictive transcripts corresponded with a shift in flagellin contributors and oxidative metabolic microenvironment, potentially factors in local CRC pathogenesis. This suggests that the effectiveness of prospective microbiome monitoring for adenomas may be limited but supports the potential causality of these consistent, early microbial changes in colonic neoplasia.
Keywords: Lynch syndrome; colorectal cancer; human microbiome; metagenomics; metatranscriptomics.
Copyright © 2020 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of interests C.H. is a member of the Seres Therapeutics scientific advisory board. A.T.C is a Stuart and Suzanne Steele MGH Research Scholar.
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References
-
- Abu-Ali GS, Mehta RS, Lloyd-Price J, Mallick H, Branck T, Ivey KL, Drew DA, DuLong C, Rimm E, Izard J, et al. (2018). Metatranscriptome of human faecal microbial communities in a cohort of adult men. Nature microbiology. 3(3), 356–366. Published online 2018/01/18 DOI: 10.1038/s41564-017-0084-4. - DOI - PMC - PubMed
-
- Abubucker S, Segata N, Goll J, Schubert AM, Izard J, Cantarel BL, Rodriguez-Mueller B, Zucker J, Thiagarajan M, Henrissat B, et al. (2012). Metabolic reconstruction for metagenomic data and its application to the human microbiome. PLoS computational biology. 8(6), e1002358 Published online 2012/06/22 DOI: 10.1371/journal.pcbi.1002358. - DOI - PMC - PubMed
-
- Albenberg L, Esipova TV, Judge CP, Bittinger K, Chen J, Laughlin A, Grunberg S, Baldassano RN, Lewis JD, Li H, et al. (2014). Correlation between intraluminal oxygen gradient and radial partitioning of intestinal microbiota. Gastroenterology. 147(5), 1055–1063.e1058. Published online 2014/07/22 DOI: 10.1053/j.gastro.2014.07.020. - DOI - PMC - PubMed
-
- Andersen-Nissen E, Smith KD, Strobe KL, Barrett SL, Cookson BT, Logan SM, and Aderem A (2005). Evasion of Toll-like receptor 5 by flagellated bacteria. Proceedings of the National Academy of Sciences of the United States of America. 102(26), 9247–9252. Published online 2005/06/16 DOI: 10.1073/pnas.0502040102. - DOI - PMC - PubMed
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