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. 2020 Jun:244:21-29.
doi: 10.1016/j.cancergen.2020.01.050. Epub 2020 Feb 10.

LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma: a pilot study

Savita Budania  1 , Debpali Sur  1 , Jitendra Nangal  2 , Sofia Pilli  1 , Koel Mukherjee  1 , Manash Biswas  3 , Ramasare Prasad  1 , Shilpi Saxena  3 , Prabhat K Mandal  4
Affiliations

LINE-1 retrotransposon encoded ORF1p expression and promoter methylation in oral squamous cell carcinoma: a pilot study

Savita Budania et al. Cancer Genet. 2020 Jun.

Abstract

Oral squamous cell carcinoma (OSCC) is highly predominant in India due to excessive use of tobacco. Here we investigated Long INterpersed Element 1 (LINE or L1) retrotransposon activity in OSCC samples in the same population. There are almost 500,000 copies of L1 occupied around 30% of the human genome. Although most of them are inactive, around 150-200 copies are actively jumping in a human genome. L1 encodes two proteins designated as ORF1p and ORF2p and expression of both proteins are critical for the process of retrotransposition. Here we have analyzed L1 ORF1p expression in a small cohort (n = 15) of paired cancer-normal tissues obtained from operated oral cancer patients. Immunohistochemistry (IHC) with the human ORF1 antibody showed the presence of ORF1p in almost 60% cancer samples, and few of them also showed aberrant p53 expression. Investigating L1 promoter methylation status, showed certain trends towards hypomethylation of the L1 promoter in cancer tissues compared to its normal counterpart. Our data raise the possibility that L1ORF1p expression might have some role in the onset and progression of this particular type of cancer.

Keywords: LINE1 ORF1p antibody; LINE1 promoter; LINE1 retrotransposon; OSCC; Oral cancer.

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Conflict of interest statement

Declaration of Competing Interest The authors declare that they have no competing interests.

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