Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer

doi:10.1016/j.bbrc.2020.02.052

. 2020 Feb 19:S0006-291X(20)30320-X.

doi: 10.1016/j.bbrc.2020.02.052. Online ahead of print.

Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer

Mingfu Tong¹, Haiming Liu², Jianyu Hao³, Daiming Fan⁴

Affiliations

¹ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.
² College of Computer Science and Technology, Jilin University, Changchun, 130012, Jilin, China.
³ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China. Electronic address: haojianyu@ccmu.edu.cn.
⁴ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China; State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Air Force Military Medical University, Xi'an, 710032, China. Electronic address: daimingfan@fmmu.edu.cn.

PMID: 32087971
DOI: 10.1016/j.bbrc.2020.02.052

Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer

Mingfu Tong et al. Biochem Biophys Res Commun. 2020.

. 2020 Feb 19:S0006-291X(20)30320-X.

doi: 10.1016/j.bbrc.2020.02.052. Online ahead of print.

Authors

Mingfu Tong¹, Haiming Liu², Jianyu Hao³, Daiming Fan⁴

Affiliations

¹ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China.
² College of Computer Science and Technology, Jilin University, Changchun, 130012, Jilin, China.
³ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China. Electronic address: haojianyu@ccmu.edu.cn.
⁴ Department of Gastroenterology, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, 100020, China; State Key Laboratory of Cancer Biology, National Clinical Research Center for Digestive Diseases and Xijing Hospital of Digestive Diseases, Air Force Military Medical University, Xi'an, 710032, China. Electronic address: daimingfan@fmmu.edu.cn.

PMID: 32087971
DOI: 10.1016/j.bbrc.2020.02.052

Abstract

Berberine (BBR), a natural isoquinoline alkaloid, has been shown to be a promising therapeutic agent for colorectal cancer (CRC), but the molecular mechanism remains unclear. Here, we used mass spectrometry-based label-free proteomics to explore the potential targets of BBR in CRC cells. Comprehensive proteomic profiles demonstrated that of 8051 identified proteins, 503 and 277 differentially expressed proteins (DEPs) were screened out of CACO2 and LOVO cells, respectively. 83 DEPs were overlapped and most of these were down-regulated. A pathway enrichment analysis pinpointed mitochondrial translation, respiratory electron transport and the citric acid (TCA) cycle as biological effectors. The data of proteomics was subsequently confirmed by citrate synthase (CS), Tu translation elongation factor (TUFM), pentatricopeptide repeat domain 3 (PTCD3) and mitochondrial ribosomal protein L48 (MRPL 48) protein measurement. CS protein expression in CRC cells and tissues was higher than it was in normal specimens. Additionally, forcible downregulation of CS led to remarkable cell proliferation inhibition. Taken together, we concluded that the anticancer effects of BBR are attributable to mitochondrial protein synthesis, TCA and respiratory electron transport inhibition and that CS might be a useful therapeutic target in CRC treatment.

Keywords: Berberine; Colorectal cancer; Label-free; Mechanism; Proteomics.

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Conflict of interest statement

Declaration of competing interest The authors declare that we don’t have any financial or associative interest that represents a conflict of interest corelated with the work submitted.

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Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer

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Comparative pharmacoproteomics reveals potential targets for berberine, a promising therapy for colorectal cancer

Authors

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Conflict of interest statement

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