Improved tools to study astrocytes

doi:10.1038/s41583-020-0264-8

Review

. 2020 Mar;21(3):121-138.

doi: 10.1038/s41583-020-0264-8. Epub 2020 Feb 10.

Improved tools to study astrocytes

Xinzhu Yu^#¹, Jun Nagai^#¹, Baljit S Khakh^{2

3}

Affiliations

¹ Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
² Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. bkhakh@mednet.ucla.edu.
³ Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. bkhakh@mednet.ucla.edu.

^# Contributed equally.

PMID: 32042146
DOI: 10.1038/s41583-020-0264-8

Review

Improved tools to study astrocytes

Xinzhu Yu et al. Nat Rev Neurosci. 2020 Mar.

. 2020 Mar;21(3):121-138.

doi: 10.1038/s41583-020-0264-8. Epub 2020 Feb 10.

Authors

Xinzhu Yu^#¹, Jun Nagai^#¹, Baljit S Khakh^{2

3}

Affiliations

¹ Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA.
² Department of Physiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. bkhakh@mednet.ucla.edu.
³ Department of Neurobiology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, USA. bkhakh@mednet.ucla.edu.

^# Contributed equally.

PMID: 32042146
DOI: 10.1038/s41583-020-0264-8

Abstract

Astrocytes are a type of glial cell that tile the CNS. They interact with multiple cell types, including neurons, glial cells and blood vessels, and are involved or implicated in brain disorders. Progress has been made in understanding astrocytes, but the field lacks detailed information concerning how they perform their multifarious functions, and how and when they influence the operations of the neural circuits with which they interact. One recognized bottleneck to progress has been the paucity of reliable tools with which to explore astrocytes within the adult vertebrate CNS in vivo. However, improved tools for molecular, genetic, morphological and physiological assessments have been developed recently or have been adapted from their original purposes to study neurons and are now being used to systematically document and interrogate astrocyte biology in vivo. These tools, their uses and limitations, and the insights that they afford are summarized in this Review.

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References

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[1] Barres, B. A. The mystery and magic of glia: a perspective on their roles in health and disease. Neuron 60, 430–440 (2008). - PubMed

[2] Barres, B. A. The mystery and magic of glia: a perspective on their roles in health and disease. Neuron 60, 430–440 (2008). - PubMed

[3] Allen, N. J. & Eroglu, C. Cell biology of astrocyte–synapse interactions. Neuron 96, 697–708 (2017). - PubMed - PMC

[4] Allen, N. J. & Eroglu, C. Cell biology of astrocyte–synapse interactions. Neuron 96, 697–708 (2017). - PubMed - PMC

[5] Eroglu, C. & Barres, B. A. Regulation of synaptic connectivity by glia. Nature 468, 223–231 (2010). - PubMed - PMC

[6] Eroglu, C. & Barres, B. A. Regulation of synaptic connectivity by glia. Nature 468, 223–231 (2010). - PubMed - PMC

[7] Khakh, B. S. & Sofroniew, M. V. Diversity of astrocyte functions and phenotypes in neural circuits. Nat. Neurosci. 18, 942–952 (2015). - PubMed - PMC

[8] Khakh, B. S. & Sofroniew, M. V. Diversity of astrocyte functions and phenotypes in neural circuits. Nat. Neurosci. 18, 942–952 (2015). - PubMed - PMC

[9] Khakh, B. S. Astrocyte–neuron interactions in the striatum: insights on identity, form, and function. Trends Neurosci. 42, 617–630 (2019). - PubMed

[10] Khakh, B. S. Astrocyte–neuron interactions in the striatum: insights on identity, form, and function. Trends Neurosci. 42, 617–630 (2019). - PubMed

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Improved tools to study astrocytes

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Improved tools to study astrocytes

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