Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988;102(1-2):19-27.
doi: 10.1007/BF01315559.

Fine structure of the vaccinia virus gene encoding the precursor of the major core protein 4 a

E Van Meir  1 , R Wittek
Affiliations

Fine structure of the vaccinia virus gene encoding the precursor of the major core protein 4 a

E Van Meir et al. Arch Virol. 1988.

Abstract

A 6008 base pair fragment of the vaccinia virus DNA containing the gene for the precursor of the major core protein 4 a, which has been designated P4 a, was sequenced. A long open reading frame (ORF) encoding a protein of molecular weight 102,157 started close to the position where the P4 a mRNA had been mapped. Analysis of the mRNA by S1 nuclease mapping and primer extension indicated that the 5' end defined by the former method is not the true 5' end. This suggests that the P4 a coding region is preceded by leader sequences that are not derived from the immediate vicinity of the gene, similar to what has been reported for another late vaccinia virus mRNA. The sequenced DNA contained several further ORFs on the same, or opposite DNA strand, providing further evidence for the close spacing of protein-coding sequences in the viral genome.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1985 Apr;82(7):2096-100 - PubMed
    1. Nucleic Acids Res. 1985 May 24;13(10):3617-33 - PubMed
    1. J Virol. 1985 Dec;56(3):830-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed
    1. Gene. 1984 Jun;28(3):351-9 - PubMed

Publication types

LinkOut - more resources