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. 1988 Nov;85(21):8081-5.
doi: 10.1073/pnas.85.21.8081.

Asynchronous DNA replication within the human beta-globin gene locus

E Epner  1 , W C Forrester, M Groudine
Affiliations

Asynchronous DNA replication within the human beta-globin gene locus

E Epner et al. Proc Natl Acad Sci U S A. 1988 Nov.

Abstract

The timing of DNA replication of the human beta-globin gene locus has been studied by blot hybridization of newly synthesized BrdUrd-substituted DNA from cells in different stages of the S phase. Using probes that span greater than 120 kilobases across the human beta-globin gene locus, we show that the majority of this domain replicates in early S phase in the human erythroleukemia cell line K562 and in middle-to-late S phase in the lymphoid cell line Manca. However, in K562 cells three small regions display a strikingly different replication pattern than adjacent sequences. These islands, located in the inter-gamma-globin gene region and approximately 20 kilobases 5' to the epsilon-globin gene and 20 kilobases 3' to the beta-globin gene, replicate later and throughout S phase. A similar area is also present in the alpha-globin gene region in K562 cells. We suggest that these regions may represent sites of termination of replication forks.

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