Titration of Igs contained in an intravenous IgM-enriched preparation against selected pathogens involved in sepsis
- PMID: 31864730
- DOI: 10.1016/j.imbio.2019.12.006
Titration of Igs contained in an intravenous IgM-enriched preparation against selected pathogens involved in sepsis
Abstract
The goal of our work was to titer the IgG, IgM and IgA in Pentaglobin® (a preparation enriched in IgM), targeting specific surface antigens of Gram-positive and Gram-negative bacteria as well as a C. albicans strain. Lipopolysaccharides from Gram-negative bacteria, peptidoglycan and lipoteichoic acid from the other microorganisms were extracted and used in several ELISA assays in order to determine the titration of immunoglobulins in Pentaglobin® directed towards the aforementioned surface antigens. Our results showed an overall immunoglobulin titer of at least 103 in Pentaglobin® with some exceptions for the IgA titers and for some immunoglobulin titers against E. faecalis, K. pneumoniae and P. aeruginosa. According to these results, Pentaglobin® can be considered as a potential adjuvant for antimicrobial therapy.
Keywords: Bloodstream infections; Immunoglobulin preparation; MDR bacteria; Titration.
Copyright © 2019 Elsevier GmbH. All rights reserved.
Conflict of interest statement
Declaration of Competing Interest The authors declare no conflict of interest.
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