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. 2019 Dec 12;179(7):1609-1622.e16.
doi: 10.1016/j.cell.2019.11.010.

Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program

Laufey Geirsdottir  1 , Eyal David  1 , Hadas Keren-Shaul  2 , Assaf Weiner  1 , Stefan Cornelius Bohlen  3 , Jana Neuber  3 , Adam Balic  4 , Amir Giladi  1 , Fadi Sheban  1 , Charles-Antoine Dutertre  5 , Christine Pfeifle  6 , Francesca Peri  7 , Antonella Raffo-Romero  8 , Jacopo Vizioli  8 , Kaspar Matiasek  9 , Christian Scheiwe  10 , Stephan Meckel  11 , Kerstin Mätz-Rensing  12 , Franziska van der Meer  12 , Finnbogi Rutur Thormodsson  13 , Christine Stadelmann  14 , Noga Zilkha  15 , Tali Kimchi  15 , Florent Ginhoux  16 , Igor Ulitsky  17 , Daniel Erny  18 , Ido Amit  19 , Marco Prinz  20
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Free article

Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program

Laufey Geirsdottir et al. Cell. .
Free article

Erratum in

  • Cross-Species Single-Cell Analysis Reveals Divergence of the Primate Microglia Program.
    Geirsdottir L, David E, Keren-Shaul H, Weiner A, Bohlen SC, Neuber J, Balic A, Giladi A, Sheban F, Dutertre CA, Pfeifle C, Peri F, Raffo-Romero A, Vizioli J, Matiasek K, Scheiwe C, Meckel S, Mätz-Rensing K, van der Meer F, Thormodsson FR, Stadelmann C, Zilkha N, Kimchi T, Ginhoux F, Ulitsky I, Erny D, Amit I, Prinz M. Geirsdottir L, et al. Cell. 2020 Apr 30;181(3):746. doi: 10.1016/j.cell.2020.04.002. Cell. 2020. PMID: 32359440 No abstract available.

Abstract

Microglia, the brain-resident immune cells, are critically involved in many physiological and pathological brain processes, including neurodegeneration. Here we characterize microglia morphology and transcriptional programs across ten species spanning more than 450 million years of evolution. We find that microglia express a conserved core gene program of orthologous genes from rodents to humans, including ligands and receptors associated with interactions between glia and neurons. In most species, microglia show a single dominant transcriptional state, whereas human microglia display significant heterogeneity. In addition, we observed notable differences in several gene modules of rodents compared with primate microglia, including complement, phagocytic, and susceptibility genes to neurodegeneration, such as Alzheimer's and Parkinson's disease. Our study provides an essential resource of conserved and divergent microglia pathways across evolution, with important implications for future development of microglia-based therapies in humans.

Keywords: immunology; microglia; neurodegeneration; single-cell RNA-seq; systems biology.

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