Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
- PMID: 31825848
- PMCID: PMC6951814
- DOI: 10.1016/j.celrep.2019.11.042
Integrated Cross-Species Analysis Identifies a Conserved Transitional Dendritic Cell Population
Abstract
Plasmacytoid dendritic cells (pDCs) are sensor cells with diverse immune functions, from type I interferon (IFN-I) production to antigen presentation, T cell activation, and tolerance. Regulation of these functions remains poorly understood but could be mediated by functionally specialized pDC subpopulations. We address pDC diversity using a high-dimensional single-cell approach: mass cytometry (CyTOF). Our analysis uncovers a murine pDC-like population that specializes in antigen presentation with limited capacity for IFN-I production. Using a multifaceted cross-species comparison, we show that this pDC-like population is the definitive murine equivalent of the recently described human AXL+ DCs, which we unify under the name transitional DCs (tDCs) given their continuum of pDC and cDC2 characteristics. tDCs share developmental traits with pDCs, as well as recruitment dynamics during viral infection. Altogether, we provide a framework for deciphering the function of pDCs and tDCs during diseases, which has the potential to open new avenues for therapeutic design.
Keywords: ASDC; AXL(+) dendritic cells; CyTOF; human; mouse; noncanonical dendritic cells; plasmacytoid dendritic cells; pre-DCs; transitional dendritic cells; viral infection.
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
Conflict of interest statement
DECLARATION OF INTERESTS
The authors declare no competing interests.
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References
-
- Alculumbre SG, Saint-André V, Di Domizio J, Vargas P, Sirven P, Bost P, Maurin M, Maiuri P, Wery M, Roman MS, et al. (2018). Diversification of human plasmacytoid predendritic cells in response to a single stimulus. Nat. Immunol 19, 63–75. - PubMed
-
- Alculumbre S, Raieli S, Hoffmann C, Chelbi R, Danlos F-X, and Soumelis V (2019). Plasmacytoid pre-dendritic cells (pDC): from molecular pathways to function and disease association. Semin. Cell Dev. Biol 86, 24–35. - PubMed
-
- Asselin-Paturel C, Boonstra A, Dalod M, Durand I, Yessaad N, Dezutter-Dambuyant C, Vicari A, O’Garra A, Biron C, Brière F, and Trinchieri G (2001). Mouse type I IFN-producing cells are immature APCs with plasmacytoid morphology. Nat. Immunol 2, 1144–1150. - PubMed
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