Cell-free gene expression: an expanded repertoire of applications

doi:10.1038/s41576-019-0186-3

Review

. 2020 Mar;21(3):151-170.

doi: 10.1038/s41576-019-0186-3. Epub 2019 Nov 28.

Cell-free gene expression: an expanded repertoire of applications

Adam D Silverman^{1

2

3}, Ashty S Karim^{1

2

3}, Michael C Jewett^{4

5

6

7

8

9}

Affiliations

¹ Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
² Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
³ Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.
⁴ Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁵ Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁶ Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁷ Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁸ Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.
⁹ Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.

PMID: 31780816
DOI: 10.1038/s41576-019-0186-3

Review

Cell-free gene expression: an expanded repertoire of applications

Adam D Silverman et al. Nat Rev Genet. 2020 Mar.

. 2020 Mar;21(3):151-170.

doi: 10.1038/s41576-019-0186-3. Epub 2019 Nov 28.

Authors

Adam D Silverman^{1

2

3}, Ashty S Karim^{1

2

3}, Michael C Jewett^{4

5

6

7

8

9}

Affiliations

¹ Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA.
² Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA.
³ Center for Synthetic Biology, Northwestern University, Evanston, IL, USA.
⁴ Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁵ Chemistry of Life Processes Institute, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁶ Center for Synthetic Biology, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁷ Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, IL, USA. m-jewett@northwestern.edu.
⁸ Robert H. Lurie Comprehensive Cancer Center, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.
⁹ Simpson Querrey Institute, Northwestern University, Chicago, IL, USA. m-jewett@northwestern.edu.

PMID: 31780816
DOI: 10.1038/s41576-019-0186-3

Abstract

Cell-free biology is the activation of biological processes without the use of intact living cells. It has been used for more than 50 years across the life sciences as a foundational research tool, but a recent technical renaissance has facilitated high-yielding (grams of protein per litre), cell-free gene expression systems from model bacteria, the development of cell-free platforms from non-model organisms and multiplexed strategies for rapidly assessing biological design. These advances provide exciting opportunities to profoundly transform synthetic biology by enabling new approaches to the model-driven design of synthetic gene networks, the fast and portable sensing of compounds, on-demand biomanufacturing, building cells from the bottom up, and next-generation educational kits.

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References

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[1] Blow, J. J. & Laskey, R. A. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. Cell 47, 577–587 (1986). - PubMed

[2] Blow, J. J. & Laskey, R. A. Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs. Cell 47, 577–587 (1986). - PubMed

[3] Fuller, R. S., Kaguni, J. M. & Kornberg, A. Enzymatic replication of the origin of the Escherichia coli chromosome. Proc. Natl Acad. Sci. USA 78, 7370–7374 (1981). - PubMed

[4] Fuller, R. S., Kaguni, J. M. & Kornberg, A. Enzymatic replication of the origin of the Escherichia coli chromosome. Proc. Natl Acad. Sci. USA 78, 7370–7374 (1981). - PubMed

[5] Preiss, T. & Hentze, M. W. Dual function of the messenger RNA cap structure in poly(A)-tail-promoted translation in yeast. Nature 392, 516–520 (1998). - PubMed

[6] Preiss, T. & Hentze, M. W. Dual function of the messenger RNA cap structure in poly(A)-tail-promoted translation in yeast. Nature 392, 516–520 (1998). - PubMed

[7] Nirenberg, M. W. & Matthaei, J. H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc. Natl Acad. Sci. USA 47, 1588–1602 (1961). - PubMed

[8] Nirenberg, M. W. & Matthaei, J. H. The dependence of cell-free protein synthesis in E. coli upon naturally occurring or synthetic polyribonucleotides. Proc. Natl Acad. Sci. USA 47, 1588–1602 (1961). - PubMed

[9] Nirenberg, M. & Leder, P. RNA codewords and protein synthesis. The effect of trinucleotides upon the binding of sRNA to ribosomes. Science 145, 1399–1407 (1964). - PubMed

[10] Nirenberg, M. & Leder, P. RNA codewords and protein synthesis. The effect of trinucleotides upon the binding of sRNA to ribosomes. Science 145, 1399–1407 (1964). - PubMed

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Cell-free gene expression: an expanded repertoire of applications

Affiliations

Cell-free gene expression: an expanded repertoire of applications

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Abstract

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Grants and funding

LinkOut - more resources

Full Text Sources

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