Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos
- PMID: 3175624
- DOI: 10.1126/science.3175624
Developmental expression of PDGF, TGF-alpha, and TGF-beta genes in preimplantation mouse embryos
Abstract
Control of growth and differentiation during mammalian embryogenesis may be regulated by growth factors from embryonic or maternal sources. With the use of single-cell messenger RNA phenotyping, the simultaneous expression of growth factor transcripts in single or small numbers of preimplantation mouse embryos was examined. Transcripts for platelet-derived growth factor A chain (PDGF-A), transforming growth factor (TGF)-alpha, and TGF-beta 1, but not for four other growth factors, were found in whole blastocysts. TGF-alpha, TGF-beta 1, and PDGF antigens were detected in blastocysts by immunocytochemistry. Both PDGF-A and TGF-alpha were detected as maternal transcripts in the unfertilized ovulated oocyte, and again in blastocysts. TGF-beta 1 transcripts appeared only after fertilization. The expression of a subset of growth factors in mouse blastocysts suggests a role for these factors in the growth and differentiation of early mammalian embryos.
Similar articles
-
Expression of growth factor ligand and receptor genes in the preimplantation bovine embryo.Mol Reprod Dev. 1992 Feb;31(2):87-95. doi: 10.1002/mrd.1080310202. Mol Reprod Dev. 1992. PMID: 1318055
-
A growth factor phenotype map for ovine preimplantation development.Biol Reprod. 1994 Apr;50(4):725-33. doi: 10.1095/biolreprod50.4.725. Biol Reprod. 1994. PMID: 7515284
-
Expression of growth factors during the differentiation of embryonic stem cells in monolayer.Dev Biol. 1990 Dec;142(2):406-13. doi: 10.1016/0012-1606(90)90362-m. Dev Biol. 1990. PMID: 2257974
-
Growth factors in preimplantation mammalian embryos.Oxf Rev Reprod Biol. 1993;15:43-81. Oxf Rev Reprod Biol. 1993. PMID: 8336981 Review.
-
[Growth factors: from oocyte maturation to blastocyst].Contracept Fertil Sex. 1994 Oct;22(10):648-55. Contracept Fertil Sex. 1994. PMID: 7804384 Review. French.
Cited by
-
Construction of spinal cord cDNA library and application for subtractive cloning of spinal cord-specific cDNAs.J Mol Neurosci. 1991;3(2):59-64. doi: 10.1007/BF02885526. J Mol Neurosci. 1991. PMID: 1687656
-
Triiodothyronine (T3) upregulates the expression of proto-oncogene TGFA independent of MAPK/ERK pathway activation in the human breast adenocarcinoma cell line, MCF7.Arch Endocrinol Metab. 2019 Mar-Apr;63(2):142-147. doi: 10.20945/2359-3997000000114. Epub 2019 Mar 21. Arch Endocrinol Metab. 2019. PMID: 30916164 Free PMC article.
-
An alternative cytoplasmic domain of the integrin beta 3 subunit.Proc Natl Acad Sci U S A. 1989 Jul;86(14):5415-8. doi: 10.1073/pnas.86.14.5415. Proc Natl Acad Sci U S A. 1989. PMID: 2787511 Free PMC article.
-
Cardiomyocyte-like cells differentiated in vitro from embryonic carcinoma cells P19 are characterized by functional expression of adrenoceptors and Ca2+ channels.In Vitro Cell Dev Biol Anim. 1994 Jul;30A(7):425-34. doi: 10.1007/BF02631310. In Vitro Cell Dev Biol Anim. 1994. PMID: 7952511
-
Immunolocalization of estrogen receptor protein in the mouse blastocyst during normal and delayed implantation.Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2376-81. doi: 10.1073/pnas.93.6.2376. Proc Natl Acad Sci U S A. 1996. PMID: 8637881 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
