TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 replication in vitro

doi:10.1016/j.rvsc.2019.10.004

. 2019 Dec:127:105-112.

doi: 10.1016/j.rvsc.2019.10.004. Epub 2019 Oct 24.

TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 replication in vitro

Huiyuan Jing¹, Wenting Ke², Ran Tao², Yang Li², Yabo Zhao², Sufang Cao³, Yanting Sun³, Jinhe Wang³, Yan Zhang³, Wang Dong³, Pandeng Zhao³, Erzhen Duan⁴

Affiliations

¹ Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China. Electronic address: lhsjhy@126.com.
² State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
³ Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
⁴ College of Biological Engineering, Henan university of technology, Zhengzhou 450001, PR China.

PMID: 31683196
DOI: 10.1016/j.rvsc.2019.10.004

TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 replication in vitro

Huiyuan Jing et al. Res Vet Sci. 2019 Dec.

. 2019 Dec:127:105-112.

doi: 10.1016/j.rvsc.2019.10.004. Epub 2019 Oct 24.

Authors

Huiyuan Jing¹, Wenting Ke², Ran Tao², Yang Li², Yabo Zhao², Sufang Cao³, Yanting Sun³, Jinhe Wang³, Yan Zhang³, Wang Dong³, Pandeng Zhao³, Erzhen Duan⁴

Affiliations

¹ Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China. Electronic address: lhsjhy@126.com.
² State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, PR China.
³ Key Laboratory of Veterinary Biological Products, College of Veterinary Medicine, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, PR China.
⁴ College of Biological Engineering, Henan university of technology, Zhengzhou 450001, PR China.

PMID: 31683196
DOI: 10.1016/j.rvsc.2019.10.004

Abstract

Porcine reproductive and respiratory syndrome (PRRS) caused by PRRS virus (PRRSV), has ranked among the major economically significant pathogen in the global swine industry. The PRRSV nonstructural protein (nsp)11 possesses nidovirus endoribonuclease (NendoU) activity, which is important for virus replication and suppression of the host innate immunity system. Recent proteomic study found that TRIM59 (tripartite motif-containing 59) interacted with the nsp11, albeit the exact role it plays in PRRSV infection remains enigmatic. Herein, we first confirmed the interaction between nsp11 and TRIM59 in co-transfected HEK293T cells and PRRSV-infected pulmonary alveolar macrophages (PAMs). The interacting domains between TRIM59 and nsp11 were further determined to be the N-terminal RING domain in TRIM59 and the C-terminal NendoU domain in nsp11, respectively. Moreover, we reported that overexpression of TRIM59 inhibited PRRSV infection in Marc-145 cells. Conversely, small interfering RNA (siRNA) depletion of TRIM59 resulted in enhanced production of PRRSV in PAMs. Together, these data add TRIM59 as a crucial antiviral component against PRRSV and provide new insights for development of new compounds to reduce PRRSV infection.

Keywords: Nonstructural protein 11 (nsp11); Porcine reproductive and respiratory syndrome virus (PRRSV); Replication; Tripartite motif 59 (TRIM59); Virus-host interactions.

PubMed Disclaimer

Cited by

TRIM Proteins in Host Defense and Viral Pathogenesis.
Giraldo MI, Hage A, van Tol S, Rajsbaum R. Giraldo MI, et al. Curr Clin Microbiol Rep. 2020;7(4):101-114. doi: 10.1007/s40588-020-00150-8. Epub 2020 Aug 8. Curr Clin Microbiol Rep. 2020. PMID: 32837832 Free PMC article. Review.
Research Progress on NSP11 of Porcine Reproductive and Respiratory Syndrome Virus.
Zheng Y, Zhang H, Luo Q, Sha H, Li G, Mu X, He Y, Kong W, Wu A, Zhang H, Yu X. Zheng Y, et al. Vet Sci. 2023 Jul 10;10(7):451. doi: 10.3390/vetsci10070451. Vet Sci. 2023. PMID: 37505856 Free PMC article. Review.
Role of transcription factors in porcine reproductive and respiratory syndrome virus infection: A review.
You X, Lei Y, Zhang P, Xu D, Ahmed Z, Yang Y. You X, et al. Front Microbiol. 2022 Jul 19;13:924004. doi: 10.3389/fmicb.2022.924004. eCollection 2022. Front Microbiol. 2022. PMID: 35928151 Free PMC article. Review.
MicroRNA-376b-3p Promotes Porcine Reproductive and Respiratory Syndrome Virus Replication by Targeting Viral Restriction Factor TRIM22.
Chen J, Zhao S, Cui Z, Li W, Xu P, Liu H, Miao X, Chen Y, Han F, Zhang H, Xia P, Zhang Y. Chen J, et al. J Virol. 2022 Jan 26;96(2):e0159721. doi: 10.1128/JVI.01597-21. Epub 2021 Nov 10. J Virol. 2022. PMID: 34757838 Free PMC article.
Research Progress in Porcine Reproductive and Respiratory Syndrome Virus-Host Protein Interactions.
Zhang H, Sha H, Qin L, Wang N, Kong W, Huang L, Zhao M. Zhang H, et al. Animals (Basel). 2022 May 27;12(11):1381. doi: 10.3390/ani12111381. Animals (Basel). 2022. PMID: 35681845 Free PMC article. Review.

See all "Cited by" articles

MeSH terms

Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions
Actions

Substances

Actions
Actions
Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
- Elsevier Science

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 replication in vitro

Affiliations

TRIM59 inhibits porcine reproductive and respiratory syndrome virus (PRRSV)-2 replication in vitro

Authors

Affiliations

Abstract

Similar articles

Cited by

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Abstract

Similar articles

Cited by

MeSH terms

Substances

Related information

LinkOut - more resources

Full Text Sources