Siderophore activity of pyoverdin for Pseudomonas aeruginosa
- PMID: 3156815
- PMCID: PMC261925
- DOI: 10.1128/iai.48.1.130-138.1985
Siderophore activity of pyoverdin for Pseudomonas aeruginosa
Abstract
Pseudomonas aeruginosa produces an extracellular compound with yellowish green fluorescence, called pyoverdin, which functions as a siderophore. The production of pyoverdin, formerly called fluorescein, is concomitant with the production of another siderophore, pyochelin. Pyoverdin is produced by P. aeruginosa in several forms, some of which were separated on gel filtration columns and on reverse-phase, high-pressure liquid chromatography columns. An active form of iron-free pyoverdin was purified to homogeneity. The elution of pyoverdin from the columns was monitored for absorbance, fluorescence, and siderophore activities. These activities, iron binding, and the stimulation of bacterial iron transport indicated that pyoverdin can function as a siderophore for P. aeruginosa. The siderophore function of pyoverdin may be related to the pathogenicity of this bacterium because pyoverdin stimulated growth not only in iron-deficient culture medium, but also in defined medium containing transferrin and in human serum or plasma.
Similar articles
-
Effects of siderophores on the growth of Pseudomonas aeruginosa in human serum and transferrin.Infect Immun. 1985 Jul;49(1):132-40. doi: 10.1128/iai.49.1.132-140.1985. Infect Immun. 1985. PMID: 3159677 Free PMC article.
-
[Pyoverdin-type siderophores from Pseudomonas aeruginosa].Z Naturforsch C J Biosci. 1986 May-Jun;41(5-6):497-506. Z Naturforsch C J Biosci. 1986. PMID: 2944297 German.
-
Synthesis and activities of pyoverdin-quinolone adducts: a prospective approach to a specific Therapy against Pseudomonas aeruginosa.J Med Chem. 2001 Jun 21;44(13):2139-51. doi: 10.1021/jm990508g. J Med Chem. 2001. PMID: 11405651
-
The pyoverdin receptor FpvA, a TonB-dependent receptor involved in iron uptake by Pseudomonas aeruginosa (review).Mol Membr Biol. 2000 Jul-Sep;17(3):123-33. doi: 10.1080/09687680050197356. Mol Membr Biol. 2000. PMID: 11128971 Review.
-
Genetics and molecular biology of siderophore-mediated iron transport in bacteria.Microbiol Rev. 1989 Dec;53(4):517-30. doi: 10.1128/mr.53.4.517-530.1989. Microbiol Rev. 1989. PMID: 2531838 Free PMC article. Review.
Cited by
-
Intraspecies Signaling between Common Variants of Pseudomonas aeruginosa Increases Production of Quorum-Sensing-Controlled Virulence Factors.mBio. 2020 Aug 25;11(4):e01865-20. doi: 10.1128/mBio.01865-20. mBio. 2020. PMID: 32843558 Free PMC article.
-
Point-of-care autofluorescence imaging for real-time sampling and treatment guidance of bioburden in chronic wounds: first-in-human results.PLoS One. 2015 Mar 19;10(3):e0116623. doi: 10.1371/journal.pone.0116623. eCollection 2015. PLoS One. 2015. PMID: 25790480 Free PMC article. Clinical Trial.
-
Outer membrane protein mediating iron uptake via pyoverdinpss, the fluorescent siderophore produced by Pseudomonas syringae pv. syringae.J Bacteriol. 1987 May;169(5):2207-14. doi: 10.1128/jb.169.5.2207-2214.1987. J Bacteriol. 1987. PMID: 3032911 Free PMC article.
-
Characterization of the pyoverdines of Azotobacter vinelandii ATCC 12837 with regard to heterogeneity.Biol Met. 1991;4(4):223-32. doi: 10.1007/BF01141185. Biol Met. 1991. PMID: 1838001
-
Impact of siderophore production on Pseudomonas aeruginosa infections in immunosuppressed mice.Infect Immun. 2000 Apr;68(4):1834-9. doi: 10.1128/IAI.68.4.1834-1839.2000. Infect Immun. 2000. PMID: 10722571 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
