Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2019 Dec 28;122(12):1359-1367.
doi: 10.1017/S0007114519002472.

Effect of high-fat diet on peripheral blood mononuclear cells and adipose tissue in early stages of diet-induced weight gain

Jake E Lowry  1 , Batbayar Tumurbaatar  2 , Claudia D'Agostino  1 , Erika Main  1 , Traver J Wright  2 , Edgar L Dillon  2 , Tais B Saito  3   4 , Craig Porter  5   6 , Clark R Andersen  7 , Douglas L Brining  1 , Janice J Endsley  3 , Melinda Sheffield-Moore  2 , Elena Volpi  2 , Rong Fang  4 , Nicola Abate  2 , Demidmaa R Tuvdendorj  2
Affiliations

Effect of high-fat diet on peripheral blood mononuclear cells and adipose tissue in early stages of diet-induced weight gain

Jake E Lowry et al. Br J Nutr. .

Abstract

Subcutaneous adipose tissue (scAT) and peripheral blood mononuclear cells (PBMC) play a significant role in obesity-associated systemic low-grade inflammation. High-fat diet (HFD) is known to induce inflammatory changes in both scAT and PBMC. However, the time course of the effect of HFD on these systems is still unknown. The aim of the present study was to determine the time course of the effect of HFD on PBMC and scAT. New Zealand white rabbits were fed HFD for 5 or 10 weeks (i.e. HFD-5 and HFD-10) or regular chow (i.e. control (CNT)-5 and CNT-10). Thereafter, metabolic and inflammatory parameters of PBMC and scAT were quantified. HFD induced hyperfattyacidaemia in HFD-5 and HFD-10 groups, with the development of insulin resistance in HFD-10, while no changes were observed in scAT lipid metabolism and inflammatory status. HFD activated the inflammatory pathways in PBMC of HFD-5 group and induced modified autophagy in that of HFD-10. The rate of fat oxidation in PBMC was directly associated with the expression of inflammatory markers and tended to inversely associate with autophagosome formation markers in PBMC. HFD affected systemic substrate metabolism, and the metabolic, inflammatory and autophagy pathways in PBMC in the absence of metabolic and inflammatory changes in scAT. Dietary approaches or interventions to avert HFD-induced changes in PBMC could be essential to prevent metabolic and inflammatory complications of obesity and promote healthier living.

Keywords: Autophagy; Low-grade inflammation; Obesogenic diet; Peripheral blood mononuclear cells; Subcutaneous adipose tissue; Weight gain.

PubMed Disclaimer

Conflict of interest statement

The authors declare no competing financial interests.

Figures

Figure 1.
Figure 1.
Western Blot images of subcutaneous adipose tissue samples. CNT, control; HFD, high fat diet; p-PLIN, phosphorylated perilipin; t-PLIN, total perilipin; adiponectin, TLR4, toll-like receptor 4.
Figure 2.
Figure 2.
Western Blot images of PBMC samples. CNT, control; HFD, high fat diet; p-p38, phosphorylated p-38; t-p38, total p-38; p-ERK1/2, phosphorylated ERK1/2; t-ERK1/2, total ERK1/2; TLR4, toll-like receptor 4; IL-1β, interleukin 1β; IL-10, interleukin 10; LC3A/B, microtubule-associated proteins 1A/1B light chain 3.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Ghanim H, Aljada A, Hofmeyer D, Syed T, Mohanty P, Dandona P. Circulating mononuclear cells in the obese are in a proinflammatory state. Circulation 110(12): 1564–71,2004. - PubMed
    1. Viardot A, Heilbronn LK, Samocha-Bonet D, Mackay F, Campbell LV, Samaras K. Obesity is associated with activated and insulin resistant immune cells. Diabetes Metab Res Rev. 28(5):447–54, 2012. - PubMed
    1. Bañuls C, Rovira-Llopis S, Lopez-Domenech S, Diaz-Morales N, Blas-Garcia A, Veses S, Morillas C, Victor VM, Rocha M, Hernandez-Mijares A. Oxidative and endoplasmic reticulum stress is impaired in leukocytes from metabolically unhealthy vs healthy obese individuals. Int J Obes (Lond). 41 (10): 1556–1563, 2017. - PubMed
    1. Wensveen FM, Valentić S, Šestan M, Turk Wensveen T, Polić B. The “Big Bang” in obese fat: Events initiating obesity-induced adipose tissue inflammation. Eur J Immunol 45(9):2446–56, 2015. - PubMed
    1. Pahlavani M, Ramalho T, Koboziev I, LeMieux MJ, Jayarathne S, Ramalingam L, Filgueiras LR, Moustaid-Moussa N. Adipose tissue inflammation in insulin resistance: review of mechanisms mediating anti-inflammatory effects of omega-3 polyunsaturated fatty acids. J Investig Med. 65(7): 1021–1027, 2017. - PMC - PubMed

Publication types