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. 1979 Aug;76(8):4061-5.
doi: 10.1073/pnas.76.8.4061.

Separation of functional subsets of human T cells by a monoclonal antibody

E L Reinherz, P C Kung, G Goldstein, S F Schlossman

Separation of functional subsets of human T cells by a monoclonal antibody

E L Reinherz et al. Proc Natl Acad Sci U S A. 1979 Aug.

Abstract

A monoclonal antibody was produced to human peripheral blood T cells. This hybridoma antibody, termed OKT4, was reactive by indirect immunofluorescence with only 55-60% of the peripheral blood T cell population (OKT4+) and unreactive with normal B cells, null cells, and macrophages. The OKT4- T cell population contained the previously described TH2+ subset that has been shown to contain cytotoxic/suppressor cells. With cell-sorter separation of OKT4+ and OKT4- cells, it was shown that these T cell subsets were functionally discrete. Both gave proliferative responses with concanavalin A, alloantigens, and phytohemagglutinin although OKT4+ cells were much more responsive to the latter. OKT4+ cells alone responded to soluble antigens whereas OKT4- cells alone were cytotoxic after alloantigenic sensitization of unfractionated T cells. However, both OKT4+ and OKT4- cells were required during sensitization for optimal development of cytotoxicity. These data suggest that the OKT4+ subset represents a helper population and that the OKT4- subset contains the cytotoxic effector population. OKT4 could be a valuable reagent for determining alterations of these functional subsets in human diseases.

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References

    1. N Engl J Med. 1979 May 10;300(19):1061-8 - PubMed
    1. J Immunol. 1979 Apr;122(4):1335-41 - PubMed
    1. J Immunol. 1978 Apr;120(4):1423-8 - PubMed
    1. J Immunol. 1978 Apr;120(4):1278-82 - PubMed
    1. J Immunol. 1979 Jul;123(1):83-6 - PubMed

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