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. 2019 Sep 9;9(9):464.
doi: 10.3390/biom9090464.

The Diagnostic Significance of PDGF, EphA7, CCR5, and CCL5 Levels in Colorectal Cancer

Muhammed Üçüncü  1 , Murat Serilmez  2 , Murat Sarı  3 , Süleyman Bademler  4 , Senem Karabulut  3
Affiliations

The Diagnostic Significance of PDGF, EphA7, CCR5, and CCL5 Levels in Colorectal Cancer

Muhammed Üçüncü et al. Biomolecules. .

Abstract

In this study, we compared the levels of C-C chemokine receptor type 5 (CCR5), C-C motif chemokine ligand 5 (CCL5), platelet-derived growth factor (PDGF), and EphrinA7 (EphA7) in patients with colorectal carcinoma and healthy controls in order to investigate the significance and usability of these potential biomarkers in early diagnosis of colorectal cancer. The study included 70 colorectal carcinoma patients and 40 healthy individuals. The CCR5, CCL5, PDGF, and EphA7 levels were measured using ELISA in blood samples. PDGF-BB, EphA7, CCR5, and CCL5 levels of the patients with colorectal carcinoma were significantly higher compared to the control group (p < 0.001 for each comparison). Our logistic regression analysis (the area under the curve was 0.958) supports the notion that PDGF-BB, EphA7, and CCL5 are potential biomarkers for the diagnosis of colon cancer. The sensitivity, specificity, and positive and negative predictive values were found to be 87.9%, 87.5%, 92.1%, and 81.4%, respectively. To our knowledge, this is the first study that investigates the relationship between colorectal carcinoma and the four biomarkers CCL5, CCR5, PDGF, and EphA7. The significantly elevated levels of all these parameters in the patient group compared to the healthy controls indicate that they can be used for the early diagnosis of colorectal carcinoma.

Keywords: CCL5; CCR5; EphA7; PDGF; chemokine; colorectal carcinoma; cytokine.

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Conflict of interest statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript, or in the decision to publish the results.

Figures

Figure 1
Figure 1
ROC curve for the combined use of markers.
Figure 2
Figure 2
PDGF-BB (ng/L) level and cancer probability.
Figure 3
Figure 3
EphA7 (ng/mL) level and cancer probability.
Figure 4
Figure 4
CCR5 (pg/mL) level and cancer probability.
Figure 5
Figure 5
CCL5 (ng/L) level and cancer probability.
See this image and copyright information in PMC

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