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. 2019 Sep 14:709:134372.
doi: 10.1016/j.neulet.2019.134372. Epub 2019 Jul 8.

Amelioration of postoperative cognitive dysfunction in mice by mesenchymal stem cell-conditioned medium treatments is associated with reduced inflammation, oxidative stress and increased BDNF expression in brain tissues

Ye Jiang  1 , Hong Gao  2 , Hongbin Yuan  3 , Haitao Xu  4 , Mouli Tian  4 , Guizhi Du  5 , Wenpin Xie  6
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Amelioration of postoperative cognitive dysfunction in mice by mesenchymal stem cell-conditioned medium treatments is associated with reduced inflammation, oxidative stress and increased BDNF expression in brain tissues

Ye Jiang et al. Neurosci Lett. .
Free article

Abstract

Objective: Mesenchymal stem cells (MSCs) are widely used in regeneration and repair of various tissues and organs, and whether MSC-conditioned medium (MSC-CM) has protective effects in postoperative cognitive dysfunction (POCD) remains largely unknown. We aimed to assess the therapeutic effect and explore the mechanisms of MSC-CM therapy in a POCD mouse model.

Methods: Sixty C57BL/6 mice were randomly assigned to 3 groups: control, POCD and POCD + MSC-CM. The POCD mouse model was established by left liver lobectomy. While mice in the control group were sham-operated, mice in the POCD + MSC-CM group were immediately administrated with MSC-CM after operation. The Morris water maze was used to determine cognitive function of mice at 1, 3, and 7 days after operation. The levels of IL-1β, IL-6, TNF-α and malondialdehyde in brain tissues at 3 days after operation were assessed by ELISA, while the protein level of brain derived neurotrophic factor (BDNF) was determined by western blot.

Results: Left liver lobectomy induced POCD in mice resulted in decrease of cognitive function, increase of brain IL-1β, IL-6, TNF-α and malondialdehyde levels, and decreased BDNF expression, while administration of MSC-CM significantly reversed these changes.

Conclusion: MSC-CM ameliorates POCD in mice, and its protective roles are associated with reduced levels of inflammatory factors, attenuated oxidative stress, and increased BDNF expression.

Keywords: Brain derived neurotrophic; Factor (BDNF); Inflammation; Mesenchymal stem cell-conditioned medium (MSC-CM); Postoperative cognitive dysfunction (POCD).

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