The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery
- PMID: 3127057
- DOI: 10.1016/0092-8674(88)90433-3
The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery
Abstract
A yeast GTP-binding protein, the YPT1 gene product, has been found to function early in the secretion pathway. The ypt1-1 mutation causes a phenotype reminiscent of early secretion-defective mutants, including accumulation of membranes and vesicles as well as a partial defect in secretion and incomplete glycosylation of invertase. Immunofluorescence localization studies using affinity-purified antibody directed against the YPT1 protein showed punctate staining of the cytoplasm of growing yeast cells and very intense staining of small buds, where membrane growth and secretion are most active. The punctate cytoplasmic staining is changed in a mutant (sec7) under conditions that cause aberrant Golgi structures to accumulate. The pattern of immunofluorescence obtained when mouse cells were stained with the antibody coincided closely with the pattern observed with wheat germ agglutinin, suggesting that a mammalian counterpart of the yeast YPT1 protein is located in the Golgi apparatus. These results are interpreted as suggesting that GTP-binding proteins may act to direct intracellular vesicle traffic.
Similar articles
-
The yeast SLY gene products, suppressors of defects in the essential GTP-binding Ypt1 protein, may act in endoplasmic reticulum-to-Golgi transport.Mol Cell Biol. 1991 Jun;11(6):2980-93. doi: 10.1128/mcb.11.6.2980-2993.1991. Mol Cell Biol. 1991. PMID: 1903839 Free PMC article.
-
Mutational analysis of the putative effector domain of the GTP-binding Ypt1 protein in yeast suggests specific regulation by a novel GAP activity.EMBO J. 1991 Apr;10(4):785-92. doi: 10.1002/j.1460-2075.1991.tb08010.x. EMBO J. 1991. PMID: 2009858 Free PMC article.
-
ADP-ribosylation factor is functionally and physically associated with the Golgi complex.Proc Natl Acad Sci U S A. 1990 Feb;87(3):1238-42. doi: 10.1073/pnas.87.3.1238. Proc Natl Acad Sci U S A. 1990. PMID: 2105501 Free PMC article.
-
The GTP-binding protein Ypt1 is required for transport in vitro: the Golgi apparatus is defective in ypt1 mutants.J Cell Biol. 1989 Sep;109(3):1015-22. doi: 10.1083/jcb.109.3.1015. J Cell Biol. 1989. PMID: 2504726 Free PMC article.
-
Two GTPase isoforms, Ypt31p and Ypt32p, are essential for Golgi function in yeast.EMBO J. 1996 Dec 2;15(23):6460-75. EMBO J. 1996. PMID: 8978673 Free PMC article.
Cited by
-
Trs130 participates in autophagy through GTPases Ypt31/32 in Saccharomyces cerevisiae.Traffic. 2013 Feb;14(2):233-46. doi: 10.1111/tra.12024. Epub 2012 Nov 21. Traffic. 2013. PMID: 23078654 Free PMC article.
-
Regulation of selective autophagy onset by a Ypt/Rab GTPase module.Proc Natl Acad Sci U S A. 2012 May 1;109(18):6981-6. doi: 10.1073/pnas.1121299109. Epub 2012 Apr 16. Proc Natl Acad Sci U S A. 2012. PMID: 22509044 Free PMC article.
-
The Small GTPases in Fungal Signaling Conservation and Function.Cells. 2021 Apr 28;10(5):1039. doi: 10.3390/cells10051039. Cells. 2021. PMID: 33924947 Free PMC article. Review.
-
Rab35 Targeting to the Plasma Membrane Is Dependent on the C-terminal Polybasic Cluster.Acta Histochem Cytochem. 2020 Aug 26;53(4):93-97. doi: 10.1267/ahc.20-00006. Epub 2020 Jul 22. Acta Histochem Cytochem. 2020. PMID: 32873993 Free PMC article.
-
Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells.J Cell Biol. 1990 Sep;111(3):987-1000. doi: 10.1083/jcb.111.3.987. J Cell Biol. 1990. PMID: 2202740 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
