Modulation of tumor necrosis factor-alpha cytotoxicity in L929 cells by bacterial toxins, hydrocortisone and inhibitors of arachidonic acid metabolism
- PMID: 3122742
- DOI: 10.1016/0006-291x(87)90440-2
Modulation of tumor necrosis factor-alpha cytotoxicity in L929 cells by bacterial toxins, hydrocortisone and inhibitors of arachidonic acid metabolism
Abstract
L929 cells were incubated with tumor necrosis factor-alpha (TNF-alpha) in the presence or absence of various inhibitors of arachidonic acid metabolism. The addition of either hydrocortisone or nordihydroguaiaretic acid (NDGA) decreased the cytotoxic effect of TNF-alpha but exogenously added arachidonate or linoleate, indomethacin and eicosatetraynoic acid (ETYA) were without effect. While it was found that TNF-alpha stimulated arachidonic acid release, no metabolites of this fatty acid could be evidenced. Cytotoxicity of TNF-alpha could also be decreased by the addition of either cholera or pertussis toxin. These results suggest that a GTP-binding protein is involved in the cytotoxic action of TNF-alpha. Arachidonic acid, released possibly by a phospholipase A2, might also play a role, but probably not via its conversion to known metabolites.
Similar articles
-
Tumour-necrosis-factor-mediated cytotoxicity is correlated with phospholipase-A2 activity, but not with arachidonic acid release per se.Eur J Biochem. 1991 Jan 30;195(2):465-75. doi: 10.1111/j.1432-1033.1991.tb15727.x. Eur J Biochem. 1991. PMID: 1847684
-
Involvement of phospholipase A2 activation in tumour cell killing by tumour necrosis factor.Immunology. 1988 May;64(1):81-5. Immunology. 1988. PMID: 3133309 Free PMC article.
-
Phospholipase A2 and phospholipase C are activated by distinct GTP-binding proteins in response to alpha 1-adrenergic stimulation in FRTL5 thyroid cells.Proc Natl Acad Sci U S A. 1986 Oct;83(19):7201-5. doi: 10.1073/pnas.83.19.7201. Proc Natl Acad Sci U S A. 1986. PMID: 3020540 Free PMC article.
-
Receptor-mediated activation of phospholipase A2 via GTP-binding proteins: arachidonic acid and its metabolites as second messengers.Trends Neurosci. 1988 Mar;11(3):117-23. doi: 10.1016/0166-2236(88)90157-9. Trends Neurosci. 1988. PMID: 2465609 Review. No abstract available.
-
Structure-function relationship of tumour necrosis factor and its mechanism of action.Ciba Found Symp. 1987;131:109-23. doi: 10.1002/9780470513521.ch8. Ciba Found Symp. 1987. PMID: 3131072 Review.
Cited by
-
The Gβγ-Src signaling pathway regulates TNF-induced necroptosis via control of necrosome translocation.Cell Res. 2014 Apr;24(4):417-32. doi: 10.1038/cr.2014.17. Epub 2014 Feb 11. Cell Res. 2014. PMID: 24513853 Free PMC article.
-
Effects of n-3 and n-6 fatty acids on tumor necrosis factor cytotoxicity in WEHI fibrosarcoma cells.Lipids. 1992 Mar;27(3):161-8. doi: 10.1007/BF02536172. Lipids. 1992. PMID: 1522759
-
Glucocorticoids as cytokine inhibitors: role in neuroendocrine control and therapy of inflammatory diseases.Mediators Inflamm. 1993;2(4):263-70. doi: 10.1155/S0962935193000365. Mediators Inflamm. 1993. PMID: 18475532 Free PMC article.
-
Streptococcal glycoprotein-induced tumour cell growth inhibition involves the modulation of a pertussis toxin-sensitive G protein.Br J Cancer. 1996 Apr;73(8):917-23. doi: 10.1038/bjc.1996.182. Br J Cancer. 1996. PMID: 8611426 Free PMC article.
-
TNF induces c-fos via a novel pathway requiring conversion of arachidonic acid to a lipoxygenase metabolite.EMBO J. 1991 Jan;10(1):109-15. doi: 10.1002/j.1460-2075.1991.tb07926.x. EMBO J. 1991. PMID: 1899225 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
