Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Sep;6(9):2617-25.
doi: 10.1002/j.1460-2075.1987.tb02552.x.

The nuclear migration signal of Xenopus laevis nucleoplasmin

T R Bürglin  1 , E M De Robertis
Affiliations

The nuclear migration signal of Xenopus laevis nucleoplasmin

T R Bürglin et al. EMBO J. 1987 Sep.

Abstract

Nucleoplasmin is the most abundant protein in the nucleus of Xenopus laevis oocytes. Its ability to target to the nucleus when microinjected into the cytoplasm has been the subject of many studies central to our understanding of how proteins segregate into nuclei. Using a cDNA clone we constructed beta-galactosidase-nucleoplasmin hybrids in modified bacterial expression vectors. The fusion proteins were expressed in Escherichia coli, purified and injected into the cytoplasm of X. laevis oocytes. The distribution of the fusion proteins between the cytoplasmic and nuclear compartments were analysed after incubation of various lengths of time. The results show that the signal sequence for nuclear transport is located close to the carboxy terminus of the protein. The signal sequence has been mapped to a small stretch of amino acids, containing a stretch of four lysines analogous to the SV40 large-T antigen signal.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Gene. 1982 Oct;19(3):269-76 - PubMed
    1. Cell. 1982 Sep;30(2):449-58 - PubMed
    1. Cell. 1983 Apr;32(4):1021-5 - PubMed
    1. J Cell Biol. 1983 May;96(5):1486-90 - PubMed
    1. Science. 1983 Nov 18;222(4625):778-82 - PubMed

Publication types