Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Sep;8(9):3619-26.
doi: 10.1128/mcb.8.9.3619-3626.1988.

RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination

R H Schiestl  1 , S Prakash
Affiliations

RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination

R H Schiestl et al. Mol Cell Biol. 1988 Sep.

Abstract

The RAD1 gene of Saccharomyces cerevisiae is required for the incision step of excision repair of damaged DNA. In this paper, we report our observations on the effect of the RAD1 gene on genetic recombination. Mitotic intrachromosomal and interchromosomal recombination in RAD+, rad1, rad52, and other rad mutant strains was examined. The rad1 deletion mutation and some rad1 point mutations reduced the frequency of intrachromosomal recombination of a his3 duplication, in which one his3 allele is deleted at the 3' end while the other his3 allele is deleted at the 5' end. Mutations in the other excision repair genes, RAD2, RAD3, and RAD4, did not lower recombination frequencies in the his3 duplication. As expected, recombination between the his3 deletion alleles in the duplication was reduced in the rad52 mutant. The frequency of HIS3+ recombinants fell synergistically in the rad1 rad52 double mutant, indicating that the RAD1 and RAD52 genes affect this recombination via different pathways. In contrast to the effect of mutations in the RAD52 gene, mutations in the RAD1 gene did not lower intrachromosomal and interchromosomal recombination between heteroalleles that carry point mutations rather than partial deletions; however, the rad1 delta mutation did lower the frequency of integration of linear plasmids and DNA fragments into homologous genomic sequences. We suggest that RAD1 plays a role in recombination after the formation of the recombinogenic substrate.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mutat Res. 1968 Nov-Dec;6(3):409-18 - PubMed
    1. J Biol Chem. 1986 Aug 5;261(22):10169-75 - PubMed
    1. Nature. 1981 Jul 23;292(5821):306-11 - PubMed
    1. Mol Cell Biol. 1983 Oct;3(10):1730-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Aug;80(15):4818-21 - PubMed

Publication types