Skip to main page content
U.S. flag

An official website of the United States government

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Apr;6(4):1005-10.
doi: 10.1002/j.1460-2075.1987.tb04852.x.

The structure of ubiquitinated histone H2B

A W Thorne, P Sautiere, G Briand, C Crane-Robinson

The structure of ubiquitinated histone H2B

A W Thorne et al. EMBO J. 1987 Apr.

Abstract

Ubiquitinated histone H2B (uH2B) has been purified from both calf and pig thymus by exclusion chromatography in 7 M urea. Digestion of uH2B with Staphylococcus aureus V8 protease yielded the peptide 114-125 containing the ubiquitin moiety. Further digestion of this peptide with trypsin removed the ubiquitin and three H2B residues from the N-terminus. Edman degradations of both peptides established that ubiquitin is attached to the epsilon-amino group of lysine 120 in both calf and pig uH2B by an iso-peptide bond to the C-terminal glycine 76 of ubiquitin.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Biochim Biophys Acta. 1974 Aug 8;359(2):341-5 - PubMed
    1. J Biol Chem. 1971 Jul 10;246(13):4206-15 - PubMed
    1. Biochem Biophys Res Commun. 1975 Aug 4;65(3):951-60 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Mar;74(3):864-8 - PubMed
    1. Proc Natl Acad Sci U S A. 1979 Dec;76(12):6386-90 - PubMed

Publication types