doi: 10.1016/0092-8674(87)90702-1.
The unusual conformation adopted by the adenine tracts in kinetoplast DNA
- PMID: 3030560
- DOI: 10.1016/0092-8674(87)90702-1
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The unusual conformation adopted by the adenine tracts in kinetoplast DNA
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Abstract
To determine the structural features responsible for the curvature of kinetoplast DNA, we studied 13 adenine tracts in Crithidia fasciculata kinetoplast DNA. The structures of the A tracts were analyzed by cutting the DNA with hydroxyl radical. Reactivity of hydroxyl radical toward the DNA backbone progressively decreased in the 5'----3' direction of each A tract. The cutting pattern of the T-rich strand was offset by 1 or 2 bp from the pattern on the A-rich strand. An A tract in a restriction fragment from plasmid pBR322 had the same cutting pattern as the kinetoplast A tracts. We interpret these experiments to show that in A tracts the width of the minor groove decreases smoothly from the 5'----3' end of the A tract.
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