doi: 10.1016/0042-6822(87)90302-3.
Expression of the poliovirus genome from infectious cDNA is dependent upon arrangements of eukaryotic and prokaryotic sequences in recombinant plasmids
- PMID: 3029989
- DOI: 10.1016/0042-6822(87)90302-3
Item in Clipboard
Expression of the poliovirus genome from infectious cDNA is dependent upon arrangements of eukaryotic and prokaryotic sequences in recombinant plasmids
Virology.
1987 Apr.
Abstract
The introduction of a cDNA copy of poliovirus type 1 (Mahoney) into cultured primate cells results in the production of infectious virus. The level of infectious virus can be increased by incorporation of eukaryotic signals of transcription and replication. We have utilized the SV40 DNA sequence coding for the early and late promoters, the SV40 origin of replication, and the enhancer elements, along with the cDNA of poliovirus, to determine the important parameters for the level of infectious virus produced following transfection. Although plasmid replication increases the level of infectivity, the major determinant of infectivity is promoter activity.
Similar articles
-
Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals.Nucleic Acids Res. 1984 Jun 25;12(12):5123-41. doi: 10.1093/nar/12.12.5123. Nucleic Acids Res. 1984. PMID: 6330698 Free PMC article.
-
Highly infectious plasmids carrying poliovirus cDNA are capable of replication in transfected simian cells.J Virol. 1986 Aug;59(2):490-3. doi: 10.1128/JVI.59.2.490-493.1986. J Virol. 1986. PMID: 3016309 Free PMC article.
-
A chimeric plasmid from cDNA clones of poliovirus and coxsackievirus produces a recombinant virus that is temperature-sensitive.Proc Natl Acad Sci U S A. 1986 Mar;83(6):1777-81. doi: 10.1073/pnas.83.6.1777. Proc Natl Acad Sci U S A. 1986. PMID: 3006071 Free PMC article.
-
Simian virus 40 late promoter region able to initiate simian virus 40 early gene transcription in the absence of the simian virus 40 origin sequence.J Virol. 1984 Apr;50(1):163-73. doi: 10.1128/JVI.50.1.163-173.1984. J Virol. 1984. PMID: 6321788 Free PMC article.
-
SV40 promoters and their regulation.Prog Nucleic Acid Res Mol Biol. 1985;32:217-36. doi: 10.1016/s0079-6603(08)60349-9. Prog Nucleic Acid Res Mol Biol. 1985. PMID: 3003799 Review. No abstract available.
Cited by
-
Linker scanning mutagenesis of the internal ribosome entry site of poliovirus RNA.J Virol. 1992 Aug;66(8):5075-86. doi: 10.1128/JVI.66.8.5075-5086.1992. J Virol. 1992. PMID: 1321289 Free PMC article.
-
Role of a viral membrane polypeptide in strand-specific initiation of poliovirus RNA synthesis.J Virol. 1991 May;65(5):2647-54. doi: 10.1128/JVI.65.5.2647-2654.1991. J Virol. 1991. PMID: 1850038 Free PMC article.
-
Enteroviral infection in end stage dilated cardiomyopathy.Klin Wochenschr. 1990 Sep 14;68(18):914-20. doi: 10.1007/BF01649038. Klin Wochenschr. 1990. PMID: 2175375
-
Addition of substitution of simian virus 40 enhancer sequences into the Moloney murine leukemia virus (M-MuLV) long terminal repeat yields infectious M-MuLV with altered biological properties.J Virol. 1988 Jul;62(7):2427-36. doi: 10.1128/JVI.62.7.2427-2436.1988. J Virol. 1988. PMID: 2836623 Free PMC article.
-
Re-localization of cellular protein SRp20 during poliovirus infection: bridging a viral IRES to the host cell translation apparatus.PLoS Pathog. 2011 Jul;7(7):e1002127. doi: 10.1371/journal.ppat.1002127. Epub 2011 Jul 14. PLoS Pathog. 2011. PMID: 21779168 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources