The ILV5 gene of Saccharomyces cerevisiae is highly expressed
- PMID: 3027658
- PMCID: PMC341325
- DOI: 10.1093/nar/14.24.9631
The ILV5 gene of Saccharomyces cerevisiae is highly expressed
Abstract
The nucleotide sequence of the yeast ILV5 gene, which codes for the branched-chain amino acid biosynthesis enzyme acetohydroxyacid reductoisomerase, has been determined. The ILV5 coding region is 1,185 nucleotides, corresponding to a polypeptide with a molecular weight of 44,280. Transcription of the ILV5 mRNA initiates at position -81 upstream from the ATG translation start codon and terminates between 218 and 222 bases downstream from the stop codon. Consensus sequences have been identified for initiation and termination of transcription, and for general control of amino acid biosynthesis, as well as repression by leucine. The ILV5 gene is regulated slightly by general amino acid control. Codon usage of the ILV5 gene has the strong bias observed in yeast genes that are highly expressed. In agreement with this, the reductoisomerase monomer, with an apparent molecular weight of 40,000, has been identified in an SDS polyacrylamide gel pattern of total soluble yeast proteins as a gene dosage dependent band.
Similar articles
-
Cloning and molecular analysis of two different ILV5 genes from a brewing strain of Saccharomyces cerevisiae.Curr Genet. 1994 Nov-Dec;26(5-6):398-402. doi: 10.1007/BF00309925. Curr Genet. 1994. PMID: 7874731
-
An enzyme in yeast mitochondria that catalyzes a step in branched-chain amino acid biosynthesis also functions in mitochondrial DNA stability.EMBO J. 1995 Jul 3;14(13):3268-76. doi: 10.1002/j.1460-2075.1995.tb07330.x. EMBO J. 1995. PMID: 7621838 Free PMC article.
-
Nucleotide sequence of the yeast ILV2 gene which encodes acetolactate synthase.Nucleic Acids Res. 1985 Jun 11;13(11):4011-27. doi: 10.1093/nar/13.11.4011. Nucleic Acids Res. 1985. PMID: 2989783 Free PMC article.
-
The RAD2 gene of Saccharomyces cerevisiae: nucleotide sequence and transcript mapping.Gene. 1985;36(3):225-34. doi: 10.1016/0378-1119(85)90177-5. Gene. 1985. PMID: 3000874
-
Yeast LEU1. Repression of mRNA levels by leucine and relationship of 5'-noncoding region to that of LEU2.J Biol Chem. 1984 Mar 25;259(6):3714-9. J Biol Chem. 1984. PMID: 6323436
Cited by
-
LEU3 of Saccharomyces cerevisiae encodes a factor for control of RNA levels of a group of leucine-specific genes.Mol Cell Biol. 1987 Aug;7(8):2708-17. doi: 10.1128/mcb.7.8.2708-2717.1987. Mol Cell Biol. 1987. PMID: 2823102 Free PMC article.
-
Expression and secretion of pea-seed lipoxygenase isoenzymes in Saccharomyces cerevisiae.Appl Microbiol Biotechnol. 1992 Jun;37(3):342-51. doi: 10.1007/BF00210990. Appl Microbiol Biotechnol. 1992. PMID: 1368907
-
Identification of novel oxidized protein substrates and physiological partners of the mitochondrial ATP-dependent Lon-like protease Pim1.J Biol Chem. 2010 Apr 9;285(15):11445-57. doi: 10.1074/jbc.M109.065425. Epub 2010 Feb 11. J Biol Chem. 2010. PMID: 20150421 Free PMC article.
-
PET genes of Saccharomyces cerevisiae.Microbiol Rev. 1990 Sep;54(3):211-25. doi: 10.1128/mr.54.3.211-225.1990. Microbiol Rev. 1990. PMID: 2215420 Free PMC article. Review.
-
Structure of yeast regulatory gene LEU3 and evidence that LEU3 itself is under general amino acid control.Nucleic Acids Res. 1987 Jul 10;15(13):5261-73. doi: 10.1093/nar/15.13.5261. Nucleic Acids Res. 1987. PMID: 3299266 Free PMC article.
References
Publication types
MeSH terms
Substances
Associated data
- Actions
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
