Uptake of GABA and nipecotic acid in astrocytes and neurons in primary cultures: changes in the sodium coupling ratio during differentiation
- PMID: 3025461
- DOI: 10.1002/jnr.490160410
Uptake of GABA and nipecotic acid in astrocytes and neurons in primary cultures: changes in the sodium coupling ratio during differentiation
Abstract
The coupling ratio between sodium and GABA or the GABA analog, (RS)- nipecotic acid, in neuronal and glial uptake of GABA or nipecotic acid was investigated as a function of the morphological differentiation of these cell types in primary cultures. Both in neurons and astrocytes a high-affinity uptake of GABA and nipecotic acid was observed regardless of the degree of differentiation. The values of Km were essentially identical in undifferentiated and differentiated cells. On the other hand, Vmax was significantly increased both in 10-day-old neurons compared to 1- and 3-day-old neurons and in astrocytes treated with dBcAMP compared to untreated cells, i.e., Vmax increased as a function of differentiation in both cell types. In neurons as well as in astrocytes the morphological differentiation resulted in an alteration in the coupling ratio between sodium and GABA. From calculated Hill coefficients it could be deduced that the coupling ratio between sodium and GABA was changed from 1 to 2 during differentiation. Similar results were obtained for (RS)-nipecotic acid.
Similar articles
-
Ion dependency of uptake and release of GABA and (RS)-nipecotic acid studied in cultured mouse brain cortex neurons.J Neurosci Res. 1983;9(3):291-302. doi: 10.1002/jnr.490090306. J Neurosci Res. 1983. PMID: 6854668
-
Comparison between (RS)-nipecotic acid and GABA transport in cultured astrocytes: coupling with two sodium ions.Neurochem Res. 1981 Mar;6(3):257-66. doi: 10.1007/BF00964041. Neurochem Res. 1981. PMID: 7279105
-
Effect of homo-beta-proline and other heterocyclic GABA analogues on GABA uptake in neurons and astroglial cells and on GABA receptor binding.J Neurochem. 1981 Dec;37(6):1509-16. doi: 10.1111/j.1471-4159.1981.tb06320.x. J Neurochem. 1981. PMID: 6278078
-
Inhibitors of the GABA uptake systems.Mol Cell Biochem. 1980 Jun 18;31(2):105-21. doi: 10.1007/BF00240816. Mol Cell Biochem. 1980. PMID: 6251361 Review.
-
Astrocytes as GABA-ergic and GABA-ceptive cells.Neurochem Res. 2012 Nov;37(11):2474-9. doi: 10.1007/s11064-012-0808-z. Epub 2012 Jun 15. Neurochem Res. 2012. PMID: 22700085 Review.
Cited by
-
Ascorbic acid transport in mouse and rat astrocytes is reversibly inhibited by furosemide, SITS, and DIDS.Neurochem Res. 1989 Dec;14(12):1169-75. doi: 10.1007/BF00965504. Neurochem Res. 1989. PMID: 2628785 Free PMC article.
-
Taurine and beta-alanine uptake in primary astrocytes differentiating in culture: effects of ions.Neurochem Res. 1988 Sep;13(9):853-8. doi: 10.1007/BF00970753. Neurochem Res. 1988. PMID: 3147403
-
High-affinity sodium-dependent uptake of ascorbic acid by rat osteoblasts.J Membr Biol. 1989 Oct;111(1):83-91. doi: 10.1007/BF01869211. J Membr Biol. 1989. PMID: 2810353
-
Astrocyte Cultures Mimicking Brain Astrocytes in Gene Expression, Signaling, Metabolism and K+ Uptake and Showing Astrocytic Gene Expression Overlooked by Immunohistochemistry and In Situ Hybridization.Neurochem Res. 2017 Jan;42(1):254-271. doi: 10.1007/s11064-016-1828-x. Epub 2016 Jan 28. Neurochem Res. 2017. PMID: 26818759
-
GABA uptake into astrocytes is not associated with significant metabolic cost: implications for brain imaging of inhibitory transmission.Proc Natl Acad Sci U S A. 2003 Oct 14;100(21):12456-61. doi: 10.1073/pnas.2132096100. Epub 2003 Oct 6. Proc Natl Acad Sci U S A. 2003. PMID: 14530410 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
