GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes
- PMID: 3015130
- DOI: 10.1016/0006-291x(86)91150-2
GTP and cytosol stimulate phosphoinositide hydrolysis in isolated platelet membranes
Abstract
Hydrolysis of polyphosphoinositides by phospholipase C was examined in isolated membranes prepared from [32P]labelled platelets. In the presence of GTP gamma S, thrombin increased the release of inositol triphosphate and inositol biphosphate approximately 500%. GTP gamma S alone stimulated release 2 fold. Maximal activation of thrombin-induced phosphoinositide hydrolysis was observed at 10 uM GTP. Although addition of calcium had no effect, 2 mM EGTA completely inhibited inositolphosphate release. Addition of high speed supernatant to [32P]labelled membranes stimulated the release of inositolphosphates. This hydrolysis was further enhanced by the addition of GTP. These data demonstrate that the breakdown of polyphosphoinositides in isolated platelet membranes is dependent on GTP and stimulated by platelet cytosol.
Similar articles
-
Activation of phospholipase C associated with isolated rabbit platelet membranes by guanosine 5'-[gamma-thio]triphosphate and by thrombin in the presence of GTP.Biochem J. 1987 Apr 15;243(2):457-65. doi: 10.1042/bj2430457. Biochem J. 1987. PMID: 2820381 Free PMC article.
-
Plasma membrane associated phospholipase C from human platelets: synergistic stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis by thrombin and guanosine 5'-O-(3-thiotriphosphate).Biochemistry. 1989 Jan 10;28(1):56-60. doi: 10.1021/bi00427a009. Biochemistry. 1989. PMID: 2539864
-
Regulation of membrane-associated and cytosolic phospholipase C activities in human platelets by guanosine triphosphate.J Biol Chem. 1986 Sep 15;261(26):11942-4. J Biol Chem. 1986. PMID: 3017954
-
Inositol lipid metabolism in the responses of stimulated platelets.Cell Calcium. 1982 Oct;3(4-5):311-22. doi: 10.1016/0143-4160(82)90019-7. Cell Calcium. 1982. PMID: 6760975 Review. No abstract available.
-
Inositol phospholipid metabolism in resting and stimulated human platelets.Cell Signal. 1992 Nov;4(6):611-7. doi: 10.1016/0898-6568(92)90042-7. Cell Signal. 1992. PMID: 1336967 Review. No abstract available.
Cited by
-
Stimulation of polyphosphoinositide hydrolysis by thrombin in membranes from human fibroblasts.Biochem J. 1987 Jul 1;245(1):49-57. doi: 10.1042/bj2450049. Biochem J. 1987. PMID: 2822018 Free PMC article.
-
Activation of phospholipase C associated with isolated rabbit platelet membranes by guanosine 5'-[gamma-thio]triphosphate and by thrombin in the presence of GTP.Biochem J. 1987 Apr 15;243(2):457-65. doi: 10.1042/bj2430457. Biochem J. 1987. PMID: 2820381 Free PMC article.
-
Specific binding of [alpha-32P]GTP to cytosolic and membrane-bound proteins of human platelets correlates with the activation of phospholipase C.Proc Natl Acad Sci U S A. 1987 Apr;84(8):2261-5. doi: 10.1073/pnas.84.8.2261. Proc Natl Acad Sci U S A. 1987. PMID: 3470789 Free PMC article.
-
Stimulation of phosphoinositide degradation and phosphatidylinositol-4-phosphate phosphorylation by GTP exclusively in plasma membrane of rat brain.Neurochem Res. 1989 Aug;14(8):717-23. doi: 10.1007/BF00964948. Neurochem Res. 1989. PMID: 2554172
-
Subcellular localization of inositol lipids in blood platelets as deduced from the use of labelled precursors.Biochem J. 1987 Jun 15;244(3):757-61. doi: 10.1042/bj2440757. Biochem J. 1987. PMID: 3446190 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
