Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites
- PMID: 3002631
- DOI: 10.1016/0092-8674(86)90761-0
Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites
Abstract
Introduction of torsional stress into active chromatin domains requires that linear DNA molecules be anchored in vivo to impede free rotation. While searching for these anchorage elements, we have localized a nuclear matrix association region (MAR) within the mouse immunoglobulin kappa gene that contains two topoisomerase II sites and is adjacent to the tissue-specific enhancer. The same matrix contact occurs when the kappa locus is in germ-line (inactive) or rear-ranged (transcribed) configurations. This constitutive anchorage site partitions the gene into V-J and C region chromatin domains. We demonstrate that at least 10,000 similar and evolutionarily conserved MAR binding sites exist in the nucleus. We propose that these sites, in association with topoisomerase II and possibly in conjunction with enhancers, play fundamental roles in the functional organization of chromatin loop domains.
Similar articles
-
High frequency of matrix attachment regions and cut-like protein x/CCAAT-displacement protein and B cell regulator of IgH transcription binding sites flanking Ig V region genes.J Immunol. 2002 Sep 1;169(5):2477-87. doi: 10.4049/jimmunol.169.5.2477. J Immunol. 2002. PMID: 12193717
-
The enhancer of the immunoglobulin heavy chain locus is flanked by presumptive chromosomal loop anchorage elements.J Biol Chem. 1987 Apr 15;262(11):5394-7. J Biol Chem. 1987. PMID: 3031052
-
Protein:DNA interactions at chromosomal loop attachment sites.Genome. 1989;31(2):503-9. doi: 10.1139/g89-098. Genome. 1989. PMID: 2561108 Review.
-
Long-range interactions between three transcriptional enhancers, active Vkappa gene promoters, and a 3' boundary sequence spanning 46 kilobases.Mol Cell Biol. 2005 Apr;25(8):3220-31. doi: 10.1128/MCB.25.8.3220-3231.2005. Mol Cell Biol. 2005. PMID: 15798207 Free PMC article.
-
Dynamic Control of Long-Range Genomic Interactions at the Immunoglobulin κ Light-Chain Locus.Adv Immunol. 2015;128:183-271. doi: 10.1016/bs.ai.2015.07.004. Epub 2015 Aug 14. Adv Immunol. 2015. PMID: 26477368 Review.
Cited by
-
p75, a polypeptide component of karyoskeletal protein-enriched fractions associated with transcriptionally active loci of Drosophila melanogaster polytene chromosomes.Mol Cell Biol. 1988 May;8(5):1877-86. doi: 10.1128/mcb.8.5.1877-1886.1988. Mol Cell Biol. 1988. PMID: 3133549 Free PMC article.
-
Protein-DNA cross-linking reveals dramatic variation in RNA polymerase II density on different histone repeats of Drosophila melanogaster.Mol Cell Biol. 1987 Sep;7(9):3341-4. doi: 10.1128/mcb.7.9.3341-3344.1987. Mol Cell Biol. 1987. PMID: 2823128 Free PMC article.
-
The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes.Mol Cell Biol. 1990 May;10(5):2302-7. doi: 10.1128/mcb.10.5.2302-2307.1990. Mol Cell Biol. 1990. PMID: 2325653 Free PMC article.
-
Yeast ARS function and nuclear matrix association coincide in a short sequence from the human HPRT locus.Mol Gen Genet. 1988 May;212(2):301-9. doi: 10.1007/BF00334700. Mol Gen Genet. 1988. PMID: 2841570
-
Intracellular forms of Drosophila topoisomerase II detected with monoclonal antibodies.Nucleic Acids Res. 1988 Nov 11;16(21):10013-23. doi: 10.1093/nar/16.21.10013. Nucleic Acids Res. 1988. PMID: 2848216 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
