Yeast mRNA splicing in vitro
- PMID: 2997224
Item in Clipboard
Yeast mRNA splicing in vitro
J Biol Chem.
.
Free article
Abstract
Synthetic actin and CYH2 pre-mRNAs containing a single intron are accurately spliced in a soluble whole cell extract of yeast. Splicing in vitro requires ATP. The excised intron is released as a lariat in which an RNA branch connects the 5' end of the molecule to the last A in the "intron conserved sequence" UACUAAC. Two other discrete RNA species produced during splicing in vitro may represent reaction intermediates: free, linear exon 1 and a form of the intron lariat extending beyond the 3' splice site to include exon 2. Both lariat forms correspond to molecules previously shown to be produced during yeast pre-mRNA splicing in vivo.
Similar articles
-
Lariat structures are in vivo intermediates in yeast pre-mRNA splicing.Cell. 1984 Dec;39(3 Pt 2):611-21. doi: 10.1016/0092-8674(84)90468-9. Cell. 1984. PMID: 6096014
-
Size and position of intervening sequences are critical for the splicing efficiency of pre-mRNA in the yeast Saccharomyces cerevisiae.Nucleic Acids Res. 1985 Jun 11;13(11):3791-804. doi: 10.1093/nar/13.11.3791. Nucleic Acids Res. 1985. PMID: 3892483 Free PMC article.
-
Molecular consequences of specific intron mutations on yeast mRNA splicing in vivo and in vitro.Cell. 1985 Aug;42(1):335-44. doi: 10.1016/s0092-8674(85)80129-x. Cell. 1985. PMID: 3893746
-
Pre-mRNA splicing.Annu Rev Genet. 1986;20:671-708. doi: 10.1146/annurev.ge.20.120186.003323. Annu Rev Genet. 1986. PMID: 2880558 Review. No abstract available.
-
On the origin of RNA splicing and introns.Cell. 1985 Sep;42(2):397-400. doi: 10.1016/0092-8674(85)90092-3. Cell. 1985. PMID: 2411416 Review. No abstract available.
Cited by
-
Early commitment of yeast pre-mRNA to the spliceosome pathway.Mol Cell Biol. 1988 Sep;8(9):3755-60. doi: 10.1128/mcb.8.9.3755-3760.1988. Mol Cell Biol. 1988. PMID: 3065622 Free PMC article.
-
Structural basis for conformational equilibrium of the catalytic spliceosome.Mol Cell. 2021 Apr 1;81(7):1439-1452.e9. doi: 10.1016/j.molcel.2021.02.021. Epub 2021 Mar 10. Mol Cell. 2021. PMID: 33705709 Free PMC article.
-
Protein interactions in nuclear pre-mRNA splicing in Saccharomyces cerevisiae.Mol Biol Rep. 1990;14(2-3):141-2. doi: 10.1007/BF00360449. Mol Biol Rep. 1990. PMID: 2141897 No abstract available.
-
Specific accessory sequences in Saccharomyces cerevisiae introns control assembly of pre-mRNAs into spliceosomes.EMBO J. 1987 Dec 1;6(12):3833-9. doi: 10.1002/j.1460-2075.1987.tb02720.x. EMBO J. 1987. PMID: 3322814 Free PMC article.
-
Effects on mRNA splicing of mutations in the 3' region of the Saccharomyces cerevisiae actin intron.Mol Cell Biol. 1987 Jan;7(1):225-30. doi: 10.1128/mcb.7.1.225-230.1987. Mol Cell Biol. 1987. PMID: 3550418 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases
