D1 protein of Drosophila melanogaster. Purification and AT-DNA binding properties
- PMID: 2997203
D1 protein of Drosophila melanogaster. Purification and AT-DNA binding properties
Abstract
D1 protein of Drosophila melanogaster is a sequence-specific DNA-binding protein which recognizes AT-rich DNA sequences. AT-rich DNA sequences in eukaryotic organisms are distributed in two characteristic ways: flanking transcriptional units and in constitutive heterochromatin. D1 could play a role in regulation of gene expression and in geographical localization of DNA sequences within the nucleus. D1 has been partially purified by ion exchange chromatography. DNA-binding activity was investigated by nucleoprotein gel electrophoresis, using end-labeled restriction fragments varying in AT sequence content. D1 binds most tightly to the satellite sequence -AATAT-, with intermediate strength to the complex satellite (359-base pair repeat) and another AT-rich (68% AT) mixed sequence DNA, and least to the simple satellite sequence -AAGAG-.
Similar articles
-
Nucleosomal structure of two Drosophila melanogaster simple satellites.J Biol Chem. 1985 Sep 25;260(21):11799-804. J Biol Chem. 1985. PMID: 3930484
-
Purification and characterization of W-protein. A DNA-binding protein showing high affinity for the W chromosome-specific repetitive DNA sequences of chicken.J Biol Chem. 1988 Sep 25;263(27):13952-61. J Biol Chem. 1988. PMID: 2843543
-
Protein D1 preferentially binds A + T-rich DNA in vitro and is a component of Drosophila melanogaster nucleosomes containing A + T-rich satellite DNA.Proc Natl Acad Sci U S A. 1982 Dec;79(23):7152-6. doi: 10.1073/pnas.79.23.7152. Proc Natl Acad Sci U S A. 1982. PMID: 6818540 Free PMC article.
-
Intraspecific diversity of nucleotide sequences within the adenine + thymine-rich region of mitochondrial DNA molecules of Drosophila mauritiana, Drosophila melanogaster and Drosophila simulans.Nucleic Acids Res. 1980 Nov 25;8(22):5391-410. doi: 10.1093/nar/8.22.5391. Nucleic Acids Res. 1980. PMID: 6258143 Free PMC article.
-
A protein that preferentially binds Drosophila satellite DNA.Proc Natl Acad Sci U S A. 1979 Feb;76(2):726-30. doi: 10.1073/pnas.76.2.726. Proc Natl Acad Sci U S A. 1979. PMID: 106393 Free PMC article.
Cited by
-
Geometry of the simian virus 40 large tumor antigen-DNA complex as probed by protease digestion.Proc Natl Acad Sci U S A. 1988 Apr;85(7):2086-90. doi: 10.1073/pnas.85.7.2086. Proc Natl Acad Sci U S A. 1988. PMID: 2832846 Free PMC article.
-
Differential protein binding in lymphocytes to a sequence in the enhancer of the mouse retrovirus SL3-3.Mol Cell Biol. 1988 Apr;8(4):1625-37. doi: 10.1128/mcb.8.4.1625-1637.1988. Mol Cell Biol. 1988. PMID: 2837650 Free PMC article.
-
Analysis of nuclear factor I binding to DNA using degenerate oligonucleotides.Nucleic Acids Res. 1986 Nov 25;14(22):9117-32. doi: 10.1093/nar/14.22.9117. Nucleic Acids Res. 1986. PMID: 3786147 Free PMC article.
-
Modification of position-effect variegation by competition for binding to Drosophila satellites.EMBO Rep. 2002 Aug;3(8):747-52. doi: 10.1093/embo-reports/kvf155. Epub 2002 Jul 15. EMBO Rep. 2002. PMID: 12151333 Free PMC article.
-
Meta-analysis reveals that genes regulated by the Y chromosome in Drosophila melanogaster are preferentially localized to repressive chromatin.Genome Biol Evol. 2013;5(1):255-66. doi: 10.1093/gbe/evt005. Genome Biol Evol. 2013. PMID: 23315381 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
