Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression
- PMID: 29920280
- DOI: 10.1016/j.devcel.2018.05.023
Arabidopsis Serrate Coordinates Histone Methyltransferases ATXR5/6 and RNA Processing Factor RDR6 to Regulate Transposon Expression
Abstract
Serrate (SE) is a key component in RNA metabolism. Little is known about whether and how it can regulate epigenetic silencing. Here, we report histone methyltransferases ATXR5 and ATXR6 (ATXR5/6) as novel partners of SE. ATXR5/6 deposit histone 3 lysine 27 monomethylation (H3K27me1) to promote heterochromatin formation, repress transposable elements (TEs), and control genome stability in Arabidopsis. SE binds to ATXR5/6-regulated TE loci and promotes H3K27me1 accumulation in these regions. Furthermore, SE directly enhances ATXR5 enzymatic activity in vitro. Unexpectedly, se mutation suppresses the TE reactivation and DNA re-replication phenotypes in the atxr5 atxr6 mutant. The suppression of TE expression results from triggering RNA-dependent RNA polymerase 6 (RDR6)-dependent RNA silencing in the se atxr5 atxr6 mutant. We propose that SE facilitates ATXR5/6-mediated deposition of the H3K27me1 mark while inhibiting RDR6-mediated RNA silencing to protect TE transcripts. Hence, SE coordinates epigenetic silencing and RNA processing machineries to fine-tune the TE expression.
Keywords: RNA silencing; chromatin modification; epigenetic; gene expression; histone methylation.
Copyright © 2018 Elsevier Inc. All rights reserved.
Similar articles
-
The role of ATXR6 expression in modulating genome stability and transposable element repression in Arabidopsis.Proc Natl Acad Sci U S A. 2022 Jan 18;119(3):e2115570119. doi: 10.1073/pnas.2115570119. Proc Natl Acad Sci U S A. 2022. PMID: 35027454 Free PMC article.
-
ATXR5 and ATXR6 are H3K27 monomethyltransferases required for chromatin structure and gene silencing.Nat Struct Mol Biol. 2009 Jul;16(7):763-8. doi: 10.1038/nsmb.1611. Epub 2009 Jun 7. Nat Struct Mol Biol. 2009. PMID: 19503079 Free PMC article.
-
DNA methyltransferases are required to induce heterochromatic re-replication in Arabidopsis.PLoS Genet. 2012 Jul;8(7):e1002808. doi: 10.1371/journal.pgen.1002808. Epub 2012 Jul 5. PLoS Genet. 2012. PMID: 22792077 Free PMC article.
-
Control of transposable elements in Arabidopsis thaliana.Chromosome Res. 2014 Jun;22(2):217-23. doi: 10.1007/s10577-014-9417-9. Chromosome Res. 2014. PMID: 24801341 Review.
-
H3K27me1 is E(z) in animals, but not in plants.Epigenetics. 2009 Aug 16;4(6):366-9. doi: 10.4161/epi.4.6.9713. Epub 2009 Aug 3. Epigenetics. 2009. PMID: 19736521 Free PMC article. Review.
Cited by
-
Beyond transcription factors: roles of mRNA decay in regulating gene expression in plants.F1000Res. 2018 Dec 17;7:F1000 Faculty Rev-1940. doi: 10.12688/f1000research.16203.1. eCollection 2018. F1000Res. 2018. PMID: 30613385 Free PMC article. Review.
-
Distinct roles of Arabidopsis ORC1 proteins in DNA replication and heterochromatic H3K27me1 deposition.Nat Commun. 2023 Mar 7;14(1):1270. doi: 10.1038/s41467-023-37024-8. Nat Commun. 2023. PMID: 36882445 Free PMC article.
-
SERRATE interacts with the nuclear exosome targeting (NEXT) complex to degrade primary miRNA precursors in Arabidopsis.Nucleic Acids Res. 2020 Jul 9;48(12):6839-6854. doi: 10.1093/nar/gkaa373. Nucleic Acids Res. 2020. PMID: 32449937 Free PMC article.
-
Oryza sativa RNA-Dependent RNA Polymerase 6 Contributes to Double-Strand Break Formation in Meiosis.Plant Cell. 2020 Oct;32(10):3273-3289. doi: 10.1105/tpc.20.00213. Epub 2020 Jul 30. Plant Cell. 2020. PMID: 32732308 Free PMC article.
-
Short Interrupted Repeat Cassette (SIRC)-Novel Type of Repetitive DNA Element Found in Arabidopsis thaliana.Int J Mol Sci. 2023 Jul 5;24(13):11116. doi: 10.3390/ijms241311116. Int J Mol Sci. 2023. PMID: 37446293 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous
