Bovine leukemia virus long terminal repeat: a cell type-specific promoter
- PMID: 2981431
- DOI: 10.1126/science.2981431
Bovine leukemia virus long terminal repeat: a cell type-specific promoter
Abstract
The functional activity of the promoter unit contained within the long terminal repeat (LTR) of bovine leukemia virus (BLV) was examined by monitoring transient expression of a heterologous gene placed under its control. Various cell lines were transfected with recombinant plasmids carrying the bacterial chloramphenicol acetyltransferase (CAT) gene coupled to the BLV LTR (pBL-cat). Transient expression of CAT activity directed by the BLV LTR was observed only in the established BLV-producer cell lines derived from fetal lamb kidney (FLK) cells and bat lung cells. The amount of CAT activity transiently expressed in FLK-BLV cells was decreased approximately tenfold by deletion of LTR sequences located within a region 100 to 170 nucleotides upstream of the RNA start site. Surprisingly, removal of the region 50 base pairs downstream of the RNA initiation site to the 3'-end of the LTR reduced the expression of CAT activity by 87 percent. The BLV LTR thus appears to be an unusual promoter unit, functioning in a cell type-specific manner and possessing sequences on both the 5' and 3' sides of the RNA start site that influence gene expression.
Similar articles
-
Two elements in the bovine leukemia virus long terminal repeat that regulate gene expression.Science. 1986 Mar 21;231(4744):1437-40. doi: 10.1126/science.3006241. Science. 1986. PMID: 3006241
-
Trans activation of the bovine leukemia virus long terminal repeat in BLV-infected cells.Science. 1985 Jan 18;227(4684):320-2. doi: 10.1126/science.2981432. Science. 1985. PMID: 2981432
-
The bovine leukemia virus X region encodes a trans-activator of its long terminal repeat.Jpn J Cancer Res. 1987 Feb;78(2):93-8. Jpn J Cancer Res. 1987. PMID: 3030987
-
Bovine leukemia: facts and hypotheses derived from the study of an infectious cancer.Adv Vet Sci Comp Med. 1988;32:149-70. doi: 10.1016/b978-0-12-039232-2.50010-4. Adv Vet Sci Comp Med. 1988. PMID: 2847501 Review.
-
Bovine leukaemia: facts and hypotheses derived from the study of an infectious cancer.Vet Microbiol. 1988 Jul;17(3):197-218. doi: 10.1016/0378-1135(88)90066-1. Vet Microbiol. 1988. PMID: 2847391 Review.
Cited by
-
In vitro methylation inhibits the promotor activity of a cloned intracisternal A-particle LTR.Nucleic Acids Res. 1986 May 27;14(10):4343-52. doi: 10.1093/nar/14.10.4343. Nucleic Acids Res. 1986. PMID: 2423967 Free PMC article.
-
Replication-induced stimulation of the major late promoter of adenovirus is correlated to the binding of a factor to sequences in the first intron.Nucleic Acids Res. 1988 May 11;16(9):3771-86. doi: 10.1093/nar/16.9.3771. Nucleic Acids Res. 1988. PMID: 2836800 Free PMC article.
-
Member of the CREB/ATF protein family, but not CREB alpha plays an active role in BLV tax trans activation in vivo.Nucleic Acids Res. 1993 Aug 11;21(16):3677-82. doi: 10.1093/nar/21.16.3677. Nucleic Acids Res. 1993. PMID: 8396235 Free PMC article.
-
Bovine immunodeficiency-like virus encodes factors which trans activate the long terminal repeat.J Virol. 1992 May;66(5):2647-52. doi: 10.1128/JVI.66.5.2647-2652.1992. J Virol. 1992. PMID: 1313891 Free PMC article.
-
Transcriptional activation encoded by the v-fos gene.Proc Natl Acad Sci U S A. 1986 May;83(10):3213-7. doi: 10.1073/pnas.83.10.3213. Proc Natl Acad Sci U S A. 1986. PMID: 3010284 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Miscellaneous
