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. 2018 Sep;163(9):2405-2413.
doi: 10.1007/s00705-018-3881-z. Epub 2018 May 19.

Analysis of genetic variability of respiratory syncytial virus groups A and B in Kuwait

Nada Madi  1 , Wassim Chehadeh  2 , Mohammed Asadzadeh  2 , Mariam Al-Turab  2 , Anfal Al-Adwani  2
Affiliations

Analysis of genetic variability of respiratory syncytial virus groups A and B in Kuwait

Nada Madi et al. Arch Virol. 2018 Sep.

Abstract

Respiratory syncytial virus (RSV) is the most frequently identified viral agent in infants, children, and elderly people with acute respiratory tract infections (ARTIs). This study is the only one of its kind in Kuwait, and its purpose was to investigate the genetic variability of the G protein gene in RSV strains prevalent in Kuwait. Respiratory samples were collected from patients with ARTIs in various hospitals in Kuwait and subjected to reverse transcription PCR (RT-PCR) amplifying a fragment of the G gene of RSV. A total of 305 samples were collected between January and mid-December 2016, and 77 (25.2%) were positive for RSV. Group A viruses were predominant over group B viruses; the RSV-A group was detected in 52 (67.5%) of the positive samples, while the RSV-B group was detected in 25 (32.5%) of the positive samples. Phylogenetic analysis showed that all RSV-A strains grouped into eight clusters of identical sequences of untyped strains. Twelve RSV-B strains, on the other hand, belonged to the RSV-B/BA10 genotype, while the rest were untyped. These data suggest that new and untyped strains of RSV-A group likely predominated in Kuwait and that the BA10 genotype of the RSV-B group became the dominant genotype in the 2016 season.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Age distribution of RSV-positive patients
Fig. 2
Fig. 2
Seasonal trends of subgroups RSV-A and RSV-B in patients with acute RTI in Kuwait from January to mid-December 2016
Fig. 3
Fig. 3
Phylogenetic tree for RSV-A (A) and RSV-B (B) nucleotide sequences from the second variable region of the G protein gene, constructed using the neighbor-joining method in MEGA version 7. Solid triangles indicate the sequences of the reference strains. Details for the reference GenBank sequences used for phylogenetic analysis are given in Table 1 and Table 2 for RSV-A and RSV-B strains, respectively. The scale bar indicates the proportion of nucleotide substitutions, and the numbers at the branches are bootstrap values determined for 1,000 repetitions. Bootstrap values greater than 50% are shown at the branches. The evolutionary distances were computed using the Tamura-Nei method and are in units of base substitutions per site
Fig. 3
Fig. 3
Phylogenetic tree for RSV-A (A) and RSV-B (B) nucleotide sequences from the second variable region of the G protein gene, constructed using the neighbor-joining method in MEGA version 7. Solid triangles indicate the sequences of the reference strains. Details for the reference GenBank sequences used for phylogenetic analysis are given in Table 1 and Table 2 for RSV-A and RSV-B strains, respectively. The scale bar indicates the proportion of nucleotide substitutions, and the numbers at the branches are bootstrap values determined for 1,000 repetitions. Bootstrap values greater than 50% are shown at the branches. The evolutionary distances were computed using the Tamura-Nei method and are in units of base substitutions per site
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