Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Aug 11;16(15):7583-600.
doi: 10.1093/nar/16.15.7583.

Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties

J M Short  1 , J M Fernandez, J A Sorge, W D Huse
Affiliations
Free PMC article

Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties

J M Short et al. Nucleic Acids Res. .
Free PMC article

Abstract

A lambda insertion type cDNA cloning vector, Lambda ZAP, has been constructed. In E. coli a phagemid, pBluescript SK(-), contained within the vector, can be excised by f1 or M13 helper phage. The excision process eliminates the need to subclone DNA inserts from the lambda phage into a plasmid by restriction digestion and ligation. This is possible because Lambda ZAP incorporates the signals for both initiation and termination of DNA synthesis from the f1 bacteriophage origin of replication (1). Six of 21 restriction sites in the excised pBluescript SK polylinker, contained within the NH2-portion of the lacZ gene, are unique in lambda ZAP. Coding sequences inserted into these restriction sites, in the appropriate reading frame, can be expressed from the lacZ promoter as fusion proteins. The features of this vector significantly increase the rate at which clones can be isolated and analyzed. The lambda ZAP vector was tested by the preparation of a chicken liver cDNA library and the isolation of actin clones by screening with oligonucleotide probes. Putative actin clones were excised from the lambda vector and identified by DNA sequencing. The ability of lambda ZAP to serve as a vector for the construction of cDNA expression libraries was determined by detecting fusion proteins from clones containing glucocerbrosidase cDNA's using rabbit IgG anti-glucocerbrosidase antibodies.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Gene. 1985;33(3):341-9 - PubMed
    1. Gene. 1985;33(1):103-19 - PubMed
    1. Proc Natl Acad Sci U S A. 1985 Nov;82(21):7289-93 - PubMed
    1. Genetics. 1986 Mar;112(3):441-57 - PubMed
    1. Gene. 1986;41(2-3):193-200 - PubMed

Publication types