The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence
- PMID: 2970061
- PMCID: PMC338355
- DOI: 10.1093/nar/16.14.7119
The Saccharomyces cerevisiae RAD18 gene encodes a protein that contains potential zinc finger domains for nucleic acid binding and a putative nucleotide binding sequence
Abstract
The RAD18 gene of Saccharomyces cerevisiae is required for postreplication repair of UV damaged DNA. We have isolated the RAD18 gene, determined its nucleotide sequence and examined if deletion mutations of this gene show different or more pronounced phenotypic effects than the previously described point mutations. The RAD18 gene open reading frame encodes a protein of 487 amino acids, with a calculated molecular weight of 55,512. The RAD18 protein contains three potential zinc finger domains for nucleic acid binding, and a putative nucleotide binding sequence that is present in many proteins that bind and hydrolyze ATP. The DNA binding and nucleotide binding activities could enable the RAD18 protein to bind damaged sites in the template DNA with high affinity. Alternatively, or in addition, RAD18 protein may be a transcriptional regulator. The rad18 deletion mutation resembles the previously described point mutations in its effects on viability, DNA repair, UV mutagenesis, and sporulation.
Similar articles
-
The structure and function of RAD6 and RAD18 DNA repair genes of Saccharomyces cerevisiae.Genome. 1989;31(2):597-600. doi: 10.1139/g89-111. Genome. 1989. PMID: 2698834 Review.
-
The Neurospora uvs-2 gene encodes a protein which has homology to yeast RAD18, with unique zinc finger motifs.Mol Gen Genet. 1993 Apr;238(1-2):225-33. doi: 10.1007/BF00279551. Mol Gen Genet. 1993. PMID: 8097557
-
Saccharomyces cerevisiae RAD5-encoded DNA repair protein contains DNA helicase and zinc-binding sequence motifs and affects the stability of simple repetitive sequences in the genome.Mol Cell Biol. 1992 Sep;12(9):3807-18. doi: 10.1128/mcb.12.9.3807-3818.1992. Mol Cell Biol. 1992. PMID: 1324406 Free PMC article.
-
Identification of RAD16, a yeast excision repair gene homologous to the recombinational repair gene RAD54 and to the SNF2 gene involved in transcriptional activation.Yeast. 1992 May;8(5):385-95. doi: 10.1002/yea.320080506. Yeast. 1992. PMID: 1626430
-
Zinc fingers and other metal-binding domains. Elements for interactions between macromolecules.J Biol Chem. 1990 Apr 25;265(12):6513-6. J Biol Chem. 1990. PMID: 2108957 Review. No abstract available.
Cited by
-
Nucleotide excision repair in Escherichia coli.Microbiol Rev. 1990 Mar;54(1):18-51. doi: 10.1128/mr.54.1.18-51.1990. Microbiol Rev. 1990. PMID: 2181258 Free PMC article. Review.
-
Domains required for dimerization of yeast Rad6 ubiquitin-conjugating enzyme and Rad18 DNA binding protein.Mol Cell Biol. 1997 Aug;17(8):4536-43. doi: 10.1128/MCB.17.8.4536. Mol Cell Biol. 1997. PMID: 9234711 Free PMC article.
-
The Hansenula polymorpha PER8 gene encodes a novel peroxisomal integral membrane protein involved in proliferation.J Cell Biol. 1995 Feb;128(3):307-19. doi: 10.1083/jcb.128.3.307. J Cell Biol. 1995. PMID: 7844145 Free PMC article.
-
High level expression and purification of herpes simplex virus type 1 immediate early polypeptide Vmw110.Nucleic Acids Res. 1991 Nov 25;19(22):6155-61. doi: 10.1093/nar/19.22.6155. Nucleic Acids Res. 1991. PMID: 1659686 Free PMC article.
-
The human RAD18 gene product interacts with HHR6A and HHR6B.Nucleic Acids Res. 2000 Jul 15;28(14):2847-54. doi: 10.1093/nar/28.14.2847. Nucleic Acids Res. 2000. PMID: 10908344 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous
