A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen
- PMID: 2959371
- DOI: 10.1016/0092-8674(87)90148-6
A human autoimmune protein associated with U1 RNA contains a region of homology that is cross-reactive with retroviral p30gag antigen
Abstract
cDNA encoding a 70 kd protein (70K) associated with U1 small nuclear ribonucleoprotein (snRNP) was cloned from a human brain-stem library using autoantibodies from patients with connective tissue disease. The cDNA-derived amino acid sequence contains 23 residues homologous to a region of murine leukemia virus group-specific antigen p30gag. The homology residues in an antigenic portion of the 70K protein and is defined by a core consensus sequence, ETPEEREERRR, that occurs as a tandem repeat in p30gag of most mammalian type C retroviruses. Anti-p30gag antibodies recognized a recombinant 70K-LacZ fusion protein as well as U1 snRNPs. Using synthetic peptides as competitors, we demonstrated that the region of homology encompasses the site of immunological cross-reactivity. Thus autoantibodies against U1 snRNPs were elicited by immunization with p30gag. On the basis of these findings, we suggest a role for retroviruses in the initiation of autoimmunity.
Similar articles
-
Determination of an epitope of the diffuse systemic sclerosis marker antigen DNA topoisomerase I: sequence similarity with retroviral p30gag protein suggests a possible cause for autoimmunity in systemic sclerosis.Proc Natl Acad Sci U S A. 1989 Nov;86(21):8492-6. doi: 10.1073/pnas.86.21.8492. Proc Natl Acad Sci U S A. 1989. PMID: 2479024 Free PMC article.
-
Human anti-nuclear ribonucleoprotein antigen autoimmune sera contain a novel subset of autoantibodies that stabilizes the molecular interaction of U1RNP-C protein with the Sm core proteins.J Immunol. 1997 May 15;158(10):5017-25. J Immunol. 1997. PMID: 9144522
-
cDNA cloning of the human U1 snRNA-associated A protein: extensive homology between U1 and U2 snRNP-specific proteins.EMBO J. 1987 Dec 1;6(12):3841-8. doi: 10.1002/j.1460-2075.1987.tb02721.x. EMBO J. 1987. PMID: 2962859 Free PMC article.
-
snRNPs and scRNPs as autoantigens: clues to the etiology of the connective tissue diseases.Clin Rheumatol. 1990 Mar;9(1 Suppl 1):10-8. doi: 10.1007/BF02205547. Clin Rheumatol. 1990. PMID: 1697517 Review. No abstract available.
-
Antigenic mimicry and autoimmune diseases.Int Rev Immunol. 1991;7(3):205-24. doi: 10.3109/08830189109061775. Int Rev Immunol. 1991. PMID: 1721932 Review.
Cited by
-
Structure-probing of U1 snRNPs gradually depleted of the U1-specific proteins A, C and 70k. Evidence that A interacts differentially with developmentally regulated mouse U1 snRNA variants.Nucleic Acids Res. 1990 Feb 11;18(3):449-57. doi: 10.1093/nar/18.3.449. Nucleic Acids Res. 1990. PMID: 2137909 Free PMC article.
-
Structure and expression of the Drosophila melanogaster gene for the U1 small nuclear ribonucleoprotein particle 70K protein.Mol Cell Biol. 1990 Jun;10(6):2492-502. doi: 10.1128/mcb.10.6.2492-2502.1990. Mol Cell Biol. 1990. PMID: 1692955 Free PMC article.
-
Are retroviruses involved in the pathogenesis of SLE? Evidence demonstrated by molecular analysis of nucleic acids from SLE patients' plasma.Rheumatol Int. 1989;9(3-5):115-21. doi: 10.1007/BF00271867. Rheumatol Int. 1989. PMID: 2692125
-
Pathogenesis of SLE: immunopathology in man.Rheumatol Int. 1991;11(3):95-100. doi: 10.1007/BF00304495. Rheumatol Int. 1991. PMID: 1754819 Review.
-
Sequential autoantigenic determinants of the small nuclear ribonucleoprotein Sm D shared by human lupus autoantibodies and MRL lpr/lpr antibodies.Clin Exp Immunol. 1994 Dec;98(3):419-26. doi: 10.1111/j.1365-2249.1994.tb05507.x. Clin Exp Immunol. 1994. PMID: 7527740 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
