Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Feb;84(3):699-703.
doi: 10.1073/pnas.84.3.699.

B protein of bacteriophage mu is an ATPase that preferentially stimulates intermolecular DNA strand transfer

A Maxwell, R Craigie, K Mizuuchi

B protein of bacteriophage mu is an ATPase that preferentially stimulates intermolecular DNA strand transfer

A Maxwell et al. Proc Natl Acad Sci U S A. 1987 Feb.

Abstract

A DNA strand-transfer reaction is an early step in the transposition of phage Mu. It has been shown that an efficient reaction in vitro requires, in addition to buffer and salt, only the Mu A protein, Mu B protein, host protein HU, ATP, and Mg2+. We have determined that, of the three protein factors involved, only the Mu B protein has an ATPase activity. The Mu B ATPase is stimulated by Mu A protein and DNA but not by either of these factors alone. Double-stranded DNA is a much better cofactor than single-stranded DNA, but there is no apparent sequence specificity. In the absence of the Mu B protein and/or ATP, the intermolecular Mu DNA strand-transfer reaction is extremely inefficient, and the strand-transfer products are predominantly the result of an intramolecular reaction. This contrasts with the efficient intermolecular reaction that occurs if Mu B protein and ATP are provided. The Mu B protein, in the presence of Mu A protein and protein HU, therefore, seems to facilitate interactions between potential DNA target sites and pairs of Mu DNA ends.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Mol Gen Genet. 1977 Mar 7;151(2):169-74 - PubMed
    1. Annu Rev Genet. 1986;20:385-429 - PubMed
    1. J Mol Biol. 1981 Aug 15;150(3):341-59 - PubMed
    1. Proc Natl Acad Sci U S A. 1982 Jul;79(14):4362-6 - PubMed
    1. Cell. 1983 Dec;35(3 Pt 2):785-94 - PubMed