Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2018 Feb;24(2):201-209.
doi: 10.3201/eid2402.171407.

Adenovirus Type 4 Respiratory Infections among Civilian Adults, Northeastern United States, 2011-20151

Adriana E Kajon, Daryl M Lamson, Camden R Bair, Xiaoyan Lu, Marie L Landry, Marilyn Menegus, Dean D Erdman, Kirsten St George

Adenovirus Type 4 Respiratory Infections among Civilian Adults, Northeastern United States, 2011-20151

Adriana E Kajon et al. Emerg Infect Dis. 2018 Feb.

Abstract

Human adenovirus type 4 (HAdV-4) is most commonly isolated in military settings. We conducted detailed molecular characterization on 36 HAdV-4 isolates recovered from civilian adults with acute respiratory disease (ARD) in the northeastern United States during 2011-2015. Specimens came from college students, residents of long-term care facilities or nursing homes, a cancer patient, and young adults without co-morbidities. HAdV-4 genome types 4a1 and 4a2, the variants most frequently detected among US military recruits in basic training before the restoration of vaccination protocols, were isolated in most cases. Two novel a-like variants were recovered from students enrolled at a college in Tompkins County, New York, USA, and a prototype-like variant distinguishable from the vaccine strain was isolated from an 18-year-old woman visiting a physician's office in Ulster County, New York, USA, with symptoms of influenza-like illness. Our data suggest that HAdV-4 might be an underestimated causative agent of ARD among civilian adults.

Keywords: ARD; HAdV-4; ILI; United States; acute respiratory disease; adenovirus; adenovirus type 4; civilians; genome typing; influenza-like illness; next-generation sequencing; outbreak; respiratory infections; viruses.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Geographic distribution of cases of human adenovirus type 4 (HAdV-4) infection identified by the New York State Department of Health through sentinel surveillance efforts targeting influenza-like illness (ILI), by HAdV-4 type, by type of civilian, by county, New York, USA, 2011–2015. Respiratory specimens were collected from patients with ILI at physicians’ offices, long-term care facilities, hospitals, and colleges and submitted to the Clinical Virology Laboratory at Wadsworth Center (Albany, New York, USA) to identify the causative agent.
Figure 2
Figure 2
In silico restriction enzyme analysis of human adenovirus type 4 genomes representing the spectrum of genetic variability of the 36 isolates characterized in study of acute respiratory infection detected in the northeastern United States, 2011–2015. We generated restriction enzyme profiles for the completely sequenced genomes obtained in this study and from reference sequences available in GenBank using Geneious Pro (31). 4p4 MIL is isolate NHR90339, 4a1 MIL is isolate NHRC3, and 4a2 MIL is isolate 42606; 4a SmaI v is isolate NY11 (GenBank accession no. KY996449) and 4a SmaI/XhoI v is isolate NY24 (GenBank accession no. KY996446). MIL, military isolate; v, variant; Vac, vaccine strain.
Figure 3
Figure 3
Phylogenetic analysis of complete genomic sequences of human adenovirus type 4 reference strains and clinical isolates representative of those examined in study of cases of acute respiratory infection detected in northeastern United States, 2011–2015. We inferred the phylogenetic tree using the maximum-likelihood method on the basis of the Kimura 2-parameter model (32). Evolutionary analyses were conducted in MEGA6 (33). Isolates sequenced in this study are in bold. GenBank accession numbers are in parentheses. Scale bar indicates substitutions per site.
See this image and copyright information in PMC

Comment in

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Hilleman MR, Werner JH. Recovery of new agent from patients with acute respiratory illness. Proc Soc Exp Biol Med. 1954;85:183–8. 10.3181/00379727-85-20825 - DOI - PubMed
    1. Hilleman MR, Werner JH, Dascomb HE, Butler RL, Stewart MT. Epidemiology of RI(RI-67) group respiratory virus infections in recruit populations. Am J Hyg. 1955;62:29–42. - PubMed
    1. Van Der Veen J, Van Der Ploeg G. An outbreak of pharyngoconjunctival fever caused by types 3 and 4 adenovirus at Waalwijk, The Netherlands. Am J Hyg. 1958;68:95–105. - PubMed
    1. Aoki K, Kato M, Ohtsuka H, Ishii K, Nakazono N, Sawada H. Clinical and aetiological study of adenoviral conjunctivitis, with special reference to adenovirus types 4 and 19 infections. Br J Ophthalmol. 1982;66:776–80. 10.1136/bjo.66.12.776 - DOI - PMC - PubMed
    1. Gomes SA, Gabbay YB, Nascimento JP, Niel C. Genome analysis of adenovirus 4a, a causative agent of pharyngoconjunctival fever and respiratory diseases in Brazil. J Med Virol. 1988;26:453–9. 10.1002/jmv.1890260413 - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources