Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Review
. 2018 Jan 18;9(1):10.
doi: 10.3390/jfb9010010.

Platelet Rich Plasma: New Insights for Cutaneous Wound Healing Management

Deborah Chicharro-Alcántara  1   2 , Mónica Rubio-Zaragoza  3   4 , Elena Damiá-Giménez  5   6 , José M Carrillo-Poveda  7   8 , Belén Cuervo-Serrato  9   10 , Pau Peláez-Gorrea  11   12 , Joaquín J Sopena-Juncosa  13   14
Affiliations
Review

Platelet Rich Plasma: New Insights for Cutaneous Wound Healing Management

Deborah Chicharro-Alcántara et al. J Funct Biomater. .

Abstract

The overall increase of chronic degenerative diseases associated with ageing makes wound care a tremendous socioeconomic burden. Thus, there is a growing need to develop novel wound healing therapies to improve cutaneous wound healing. The use of regenerative therapies is becoming increasingly popular due to the low-invasive procedures needed to apply them. Platelet-rich plasma (PRP) is gaining interest due to its potential to stimulate and accelerate the wound healing process. The cytokines and growth factors forming PRP play a crucial role in the healing process. This article reviews the emerging field of skin wound regenerative therapies with particular emphasis on PRP and the role of growth factors in the wound healing process.

Keywords: growth factors; platelet-rich plasma; skin; stem cells; wound healing.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Growth factors and cytokines involved in the wound healing process (G-CSF: Granulocyte colony stimulating factor; TGF: Transforming growth factor; MMPS: Matrix metalloproteinases; TIMPS: Tissue inhibitor of MMP; IL: Interleukin; PDGF: Platelet derived growth factor; FGF: Fibroblast growth factor; VEGF: Vascular endothelial growth factor; EGF: Epidermal growth factor; KGF: Keratinocyte growth factor; GM-CSF: Granulocyte macrophage colony stimulating factor).
Figure 2
Figure 2
Methodology for PRGF® preparation. (A) After blood collection under sterile conditions in sodium citrate tubes 3.8% (PRGF® collection tube 5 mL, BTI Biotechnology Institute, Álava, Spain), tubes are centrifuged at 460× g during 8 min (PRGF® System, BTI Biotechnology Institute, Álava, Spain); (B) Blood separation into three fractions: plasma containing mostly platelets (top layer), the white blood cell layer “buffy coat” (middle layer) and red blood cells (bottom layer); (C) Pipetting procedure under a laminar flow Hood; (D,E) The Plasma Poor in Growth Factors fraction (PPGF-top part of first fraction) and Plasma Rich in Growth Factors fraction (PRGF-just above the “buffy coat”) are transferred to individual fractionation tubes with no additive (PRGF® Fractionation tubes, BTI Biotechnology Institute, Álava, Spain); (F) PRGF® activator (calcium chloride) is added to the PRGF preparation (50 μL PRGF activator® per mL of preparation) to achieve platelet degranulation and release of growth factors.
Figure 3
Figure 3
Plasma Rich in Growth Factors (PRGF) therapeutic formulations. (A) Liquid PRGF in a canine cutaneous ulcer; (B) Scaffold-like PRGF mixed with spongy bone tissue; (C) PRGF fibrin membrane for traumatic wound during orthopaedic surgery.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Crovetti G., Martinelli G., Issi M., Barone M., Guizzardi M., Campanati B., Moroni M., Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus. Apher. Sci. 2004;30:145–151. doi: 10.1016/j.transci.2004.01.004. - DOI - PubMed
    1. Theoret C. Tissue engineering in wound repair: The three “R”s—Repair, replace, regenerate. Vet. Surg. 2009;38:905–913. doi: 10.1111/j.1532-950X.2009.00585.x. - DOI - PubMed
    1. Hassan W.U., Greiser U., Wang W. Role of adipose-derived stem cells in wound healing. Wound Repair Regen. 2014;22:313–325. doi: 10.1111/wrr.12173. - DOI - PubMed
    1. Teng M., Huang Y., Zhang H. Application of stems cells in wound Healing—An update. Wound Repair Regen. 2014;22:151–160. doi: 10.1111/wrr.12152. - DOI - PubMed
    1. Singer A.J., Clark R.A. Cutaneous wound healing. N. Engl. J. Med. 1999;341:738–746. doi: 10.1056/NEJM199909023411006. - DOI - PubMed

LinkOut - more resources