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. 2018 Apr;20(1):84-89.
doi: 10.22074/cellj.2018.4915. Epub 2017 Dec 1.

miR-31 and miR-145 as Potential Non-Invasive Regulatory Biomarkers in Patients with Endometriosis

Oranous Bashti  1 , Mehrdad Noruzinia  2 , Masoud Garshasbi  1 , Morteza Abtahi  3
Affiliations

miR-31 and miR-145 as Potential Non-Invasive Regulatory Biomarkers in Patients with Endometriosis

Oranous Bashti et al. Cell J. 2018 Apr.

Erratum in

Abstract

Objectives: Endometriosis is a prevalent gynecologic disease affecting 10% of women in reproductive age. Endometriosis is diagnosed by laparoscopy that was followed by histologic confirmation. Early diagnosis will lead to a more effective treatment with much less morbidity. As miR-31 and miR-145 are shown to be directly or indirectly correlated to biological processes involved in endometriosis, the aim of this study was to examine the association of miR-31 and miR-145 expression in plasma with the presence of endometriosis.

Materials and methods: In this case control study, the plasma samples of 55 patients with endometriosis and 23 women without endometriosis were collected, extracted and analyzed by real time quantitative polymerase chain reaction (qPCR) for the expression of miR-145 and miR-31.

Results: Our findings showed that miR-31 expression levels in stage 3 or 4 and stage 1 or 2 were significantly downregulated (less than 0.01-fold, P<0.05), while the expression level of miR-145 was significantly up-regulated in women with endometriosis in stage 1 or 2.

Conclusions: Different cellular biological processes, such as differentiation, proliferation, mitochondrial function, reactive oxygen species (ROS) production, invasion and decidualization, are deregulated in endometriosis. miR-31 and miR-145 are microRNAs (miRNAs) with potential roles, as shown in pathologies like cancers. We found that miR- 31 was under-expressed in patients with endometriosis, while miR-145 was over-expressed in stage 1 or 2, indicating that they were relatively down-regulated in the more severe forms. Our findings suggested that these two miRNAs may be considered as potential biomarkers with probable implications in early diagnosis and even follow-up of patients with endometriosis.

Keywords: Biomarker; Endometriosis; miR-145; miR-31; miRNA.

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Conflict of interest statement

There is no conflict of interest in this study.

Figures

Fig.1
Fig.1
Standard curves. A. miR-31, B. miR-145, and C. miR-103-3p. Efficiencies of amplifications are 97.3 (R2=0.98), 101.8 (R2=0.974) and 100.958 (R2=0.999), respectively. R2; The coefficient of correlation and Ct; Cycle threshold.
Fig.2
Fig.2
Melting curves of quantitative polymerase chain reactions (qPCR) of miR-31 and miR145. There is no nonspecific or significant dimmer primer peak confirming the specificity of reactions.
Fig.3
Fig.3
Expression analysis of miR-31 and miR-145 in patients with endometriosisstage 1 or 2 and stage 3 or 4. Relative expressions of different groups are shownin comparison with normal controls. A. The relative expression of miR-31 in stages1 or 2 and stages 3 or 4 compared with Normal and B. The relative expression ofmiR-145 in stages 1 or 2 and stages 3 or 4 compared with normal. In stage 1 or2, the expression level of miR-145 was increased by 6.7-fold (P<0.05), whereas theexpression level of miR-31 was decreased [relative quantity (RQ)<0.01, P<0.05)]. Instage 3 or 4, the expression level of miR-145 was increased by 1.4-fold, suggestingthis change did not reach to a statistical significance level. The expression level of miR-31 was decreased as compared to normal controls (RQ<0.01, P<0.05).
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References

    1. Kao L, Germeyer A, Tulac S, Lobo S, Yang J, Taylor R, et al. Expression profiling of endometrium from women with endometriosis reveals candidate genes for disease-based implantation failure and infertility. Endocrinology. 2003;144(7):2870–2881. - PubMed
    1. Burney RO, Talbi S, Hamilton AE, Vo KC, Nyegaard M, Nezhat CR, et al. Gene expression analysis of endometrium reveals progesterone resistance and candidate susceptibility genes in women with endometriosis. Endocrinology. 2007;148(8):3814–3826. - PubMed
    1. Dyson MT, Roqueiro D, Monsivais D, Ercan CM, Pavone ME, Brooks DC, et al. Genome-wide DNA methylation analysis predicts an epigenetic switch for GATA factor expression in endometriosis. PLoS Genet. 2014;10(3):e1004158–e1004158. - PMC - PubMed
    1. Klemmt PA, Carver JG, Kennedy SH, Koninckx PR, Mardon HJ. Stromal cells from endometriotic lesions and endometrium from women with endometriosis have reduced decidualization capacity. Fertil Steril. 2006;85(3):564–572. - PMC - PubMed
    1. Wei Q, St Clair JB, Fu T, Stratton P, Nieman LK. Reduced expression of biomarkers associated with the implantation window in women with endometriosis. Fertil Steril. 2009;91(5):1686–16891. - PMC - PubMed

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