The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling
- PMID: 29117567
- DOI: 10.1016/j.celrep.2017.10.055
The Host Protein Reticulon 3.1A Is Utilized by Flaviviruses to Facilitate Membrane Remodelling
Abstract
Flaviviruses are enveloped, positive-sensed single-stranded RNA viruses that remodel host membranes, incorporating both viral and host factors facilitating viral replication. In this study, we identified a key role for the membrane-bending host protein Reticulon 3.1 (RTN3.1A) during the replication cycle of three flaviviruses: West Nile virus (WNV), Dengue virus (DENV), and Zika virus (ZIKV). We observed that, during infection, RTN3.1A is redistributed and recruited to the viral replication complex, a recruitment facilitated via the WNV NS4A protein, however, not DENV or ZIKV NS4A. Critically, small interfering RNA (siRNA)-mediated knockdown of RTN3.1A expression attenuated WNV, DENV, and ZIKV replication and severely affected the stability and abundance of the NS4A protein, coinciding with a significant alternation and reduction of viral membrane structures in the endoplasmic reticulum. These observations identified a crucial role of RTN3.1A for the viral remodelling of host membranes during efficient flavivirus replication and the stabilization of viral proteins within the endoplasmic reticulum.
Keywords: Dengue virus; NS4A; West Nile virus; Zika virus; flavivirus; host-virus interaction; membrane; reticulon; virus replication.
Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
Similar articles
-
Dengue and Zika viruses subvert reticulophagy by NS2B3-mediated cleavage of FAM134B.Autophagy. 2017 Feb;13(2):322-332. doi: 10.1080/15548627.2016.1265192. Epub 2017 Jan 19. Autophagy. 2017. PMID: 28102736 Free PMC article.
-
BPIFB3 Regulates Endoplasmic Reticulum Morphology To Facilitate Flavivirus Replication.J Virol. 2020 Apr 16;94(9):e00029-20. doi: 10.1128/JVI.00029-20. Print 2020 Apr 16. J Virol. 2020. PMID: 32102874 Free PMC article.
-
Zika Virus Is Not Uniquely Stable at Physiological Temperatures Compared to Other Flaviviruses.mBio. 2016 Sep 6;7(5):e01396-16. doi: 10.1128/mBio.01396-16. mBio. 2016. PMID: 27601578 Free PMC article.
-
Establishment and Application of Flavivirus Replicons.Adv Exp Med Biol. 2018;1062:165-173. doi: 10.1007/978-981-10-8727-1_12. Adv Exp Med Biol. 2018. PMID: 29845532 Review.
-
Structural biology of Zika virus and other flaviviruses.Nat Struct Mol Biol. 2018 Jan;25(1):13-20. doi: 10.1038/s41594-017-0010-8. Epub 2018 Jan 8. Nat Struct Mol Biol. 2018. PMID: 29323278 Review.
Cited by
-
Compartmentalized replication organelle of flavivirus at the ER and the factors involved.Cell Mol Life Sci. 2021 Jun;78(11):4939-4954. doi: 10.1007/s00018-021-03834-6. Epub 2021 Apr 12. Cell Mol Life Sci. 2021. PMID: 33846827 Free PMC article. Review.
-
"Make way": Pathogen exploitation of membrane traffic.Curr Opin Cell Biol. 2020 Aug;65:78-85. doi: 10.1016/j.ceb.2020.02.011. Epub 2020 Mar 29. Curr Opin Cell Biol. 2020. PMID: 32234681 Free PMC article. Review.
-
Flavivirus Replication Organelle Biogenesis in the Endoplasmic Reticulum: Comparison with Other Single-Stranded Positive-Sense RNA Viruses.Int J Mol Sci. 2019 May 11;20(9):2336. doi: 10.3390/ijms20092336. Int J Mol Sci. 2019. PMID: 31083507 Free PMC article. Review.
-
Eating the unknown: Xenophagy and ER-phagy are cytoprotective defenses against pathogens.Exp Cell Res. 2020 Nov 1;396(1):112276. doi: 10.1016/j.yexcr.2020.112276. Epub 2020 Sep 9. Exp Cell Res. 2020. PMID: 32918896 Free PMC article. Review.
-
Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes.PLoS One. 2020 Sep 17;15(9):e0239153. doi: 10.1371/journal.pone.0239153. eCollection 2020. PLoS One. 2020. PMID: 32941510 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
Molecular Biology Databases
