Endocytic pathway of recombinant murine tumor necrosis factor in L-929 cells
Affiliations
- PMID: 2902145
Item in Clipboard
Endocytic pathway of recombinant murine tumor necrosis factor in L-929 cells
J Immunol.
.
Abstract
The fate of TNF after binding to the surface of L-929 cells was followed by using murine rTNF coupled to colloidal gold as a probe. A time-course study using electron microscopy was performed. Our results confirm previous indications obtained from biochemical studies suggesting that TNF is internalized by this cell type. They further directly show that internalization proceeds through the classical receptor-mediated endocytosis pathway, i.e., via clathrin-coated structures and endosomes before accumulation in secondary lysosomes.
Similar articles
-
Platelet-derived growth factor: morphologic and biochemical studies of binding, internalization, and degradation.J Cell Physiol. 1984 Nov;121(2):263-74. doi: 10.1002/jcp.1041210202. J Cell Physiol. 1984. PMID: 6092390
-
Internalization and recycling of glycoprotein 280 in BN/MSV yolk sac epithelial cells: a model system of relevance to receptor-mediated endocytosis in the renal proximal tubule.Exp Nephrol. 1997 Sep-Oct;5(5):375-83. Exp Nephrol. 1997. PMID: 9386973
-
Transport of residual endocytosed products into terminal lysosomes occurs slowly in rat liver endothelial cells.Hepatology. 1998 Nov;28(5):1378-89. doi: 10.1002/hep.510280529. Hepatology. 1998. PMID: 9794925
-
A clathrin, caveolae, and dynamin-independent endocytic pathway requiring free membrane cholesterol drives HIV-1 internalization and infection in polarized trophoblastic cells.J Mol Biol. 2007 May 18;368(5):1267-83. doi: 10.1016/j.jmb.2007.03.003. Epub 2007 Mar 12. J Mol Biol. 2007. PMID: 17395200
-
Basic fibroblast growth factor (FGF-2) internalization through the heparan sulfate proteoglycans-mediated pathway: an ultrastructural approach.Eur J Cell Biol. 1995 Jan;66(1):47-59. Eur J Cell Biol. 1995. PMID: 7750519
Cited by
-
Six distinct NFκB signaling codons convey discrete information to distinguish stimuli and enable appropriate macrophage responses.Immunity. 2021 May 11;54(5):916-930.e7. doi: 10.1016/j.immuni.2021.04.011. Immunity. 2021. PMID: 33979588 Free PMC article.
-
α4/α9 Integrins Coordinate Epithelial Cell Migration Through Local Suppression of MAP Kinase Signaling Pathways.Front Cell Dev Biol. 2021 Nov 25;9:750771. doi: 10.3389/fcell.2021.750771. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34900996 Free PMC article.
-
TNF-α downregulates inhibitory neurotransmission through protein phosphatase 1-dependent trafficking of GABA(A) receptors.J Neurosci. 2013 Oct 2;33(40):15879-93. doi: 10.1523/JNEUROSCI.0530-13.2013. J Neurosci. 2013. PMID: 24089494 Free PMC article.
-
TNF is necessary for castration-induced prostate regression, whereas TRAIL and FasL are dispensable.Mol Endocrinol. 2011 Apr;25(4):611-20. doi: 10.1210/me.2010-0312. Epub 2011 Feb 3. Mol Endocrinol. 2011. PMID: 21292828 Free PMC article.
-
Structural insights of homotypic interaction domains in the ligand-receptor signal transduction of tumor necrosis factor (TNF).BMB Rep. 2016 Mar;49(3):159-66. doi: 10.5483/bmbrep.2016.49.3.205. BMB Rep. 2016. PMID: 26615973 Free PMC article. Review.