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. 2017 Sep;14(3):1905-1908.
doi: 10.3892/etm.2017.4760. Epub 2017 Jul 10.

GFAP expression in injured astrocytes in rats

Shuquan Zhang  1 , Minfei Wu  2 , Chuangang Peng  2 , Guanjie Zhao  3 , Rui Gu  4
Affiliations

GFAP expression in injured astrocytes in rats

Shuquan Zhang et al. Exp Ther Med. 2017 Sep.

Abstract

Glial fibrillary acidic protein (GFAP) is one of the best markers for the activation of astrocytes (AS) following injury or stress in the central nervous system (CNS). The purpose of this study was to examine the expression of GFAP and 14-3-3ε in rat AS subjected to hypoxia. We established primary cultures of AS from cerebral cortex of neonatal Sprague-Dawley rats as a model of glucose deficiency and hypoxia/ischemia-reperfusion. We analyzed the activated astrocyte markers GFAP and 14-3-3ε by western blot analysis and found that both increased over time, starting at 4 h and reaching the highest level at 72 h, at the end of the experiment. GFAP and 14-3-3ε protein localization by double-labeling immunofluorescence showed elevated expression and co-localization in the cytoplasm of AS. GFAP and 14-3-3ε expression remained elevated in AS 72 h after stress conditions, which is possibly related to the excessive activation and dysfunction of the CNS in chronic injuries.

Keywords: 14-3-3ε protein; astrocytes; glial fibrillary acidic protein.

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Figures

Figure 1.
Figure 1.
Expression of GFAP and 14-3-3ε by western blot analysis at different time-points. GFAP, glial fibrillary acidic protein.
Figure 2.
Figure 2.
Quantification of western blot analysis data for GFAP and 14-3-3ε expression at different time-points. GFAP, glial fibrillary acidic protein.
Figure 3.
Figure 3.
Double-labeling immunofluorescence of GFAP and 14-3-3ε in astrocytes subjected to hypoxia for different times. GFAP (green), 14-3-3ε (red), and Hoechst (blue). All images were collected at ×400 magnification. GFAP, glial fibrillary acidic protein.
See this image and copyright information in PMC

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