doi: 10.1016/0005-2728(87)90043-0.
Saturation-transfer studies of ATP-Pi exchange in isolated perfused rat liver
- PMID: 2887203
- DOI: 10.1016/0005-2728(87)90043-0
Item in Clipboard
Saturation-transfer studies of ATP-Pi exchange in isolated perfused rat liver
Biochim Biophys Acta.
.
Abstract
The rate of exchange between inorganic phosphate and ATP was measured in isolated perfused rat livers in the direction of ATP synthesis using 31P NMR spectroscopy and the saturation-transfer technique. Measurement of ATP hydrolysis was not observable, even after treatment of rats with 100 micrograms T3/day per 100 g body wt. When the perfused livers were treated with iodoacetate in order to inhibit glycolysis, NMR measurable exchange between ATP and Pi was eliminated. It is concluded that the inorganic phosphate----ATP conversion detected by saturation transfer is catalyzed by enzymes of the glycolytic pathway and that the mitochondrial ATPase rate is too slow to contribute to the observed effect.
Similar articles
-
31P-NMR saturation transfer measurements of exchange between Pi and ATP in the reactions catalysed by glyceraldehyde-3-phosphate dehydrogenase and phosphoglycerate kinase in vitro.Biochim Biophys Acta. 1987 Apr 2;928(1):45-55. doi: 10.1016/0167-4889(87)90084-x. Biochim Biophys Acta. 1987. PMID: 3828401
-
31P NMR studies of ATP synthesis and hydrolysis kinetics in the intact myocardium.Biochemistry. 1987 Nov 17;26(23):7501-10. doi: 10.1021/bi00397a045. Biochemistry. 1987. PMID: 3427090
-
31P NMR saturation-transfer measurements in Saccharomyces cerevisiae: characterization of phosphate exchange reactions by iodoacetate and antimycin A inhibition.Biochemistry. 1987 Nov 17;26(23):7483-92. doi: 10.1021/bi00397a043. Biochemistry. 1987. PMID: 3322400
-
What do magnetic resonance-based measurements of Pi→ATP flux tell us about skeletal muscle metabolism?Diabetes. 2012 Aug;61(8):1927-34. doi: 10.2337/db11-1725. Diabetes. 2012. PMID: 22826313 Free PMC article. Review.
-
Probing the properties of enzymes in vivo using NMR.Biochem Soc Trans. 1995 May;23(2):376-81. doi: 10.1042/bst0230376. Biochem Soc Trans. 1995. PMID: 7672407 Review. No abstract available.
Cited by
-
Measurement of unidirectional Pi to ATP flux in human visual cortex at 7 T by using in vivo 31P magnetic resonance spectroscopy.Proc Natl Acad Sci U S A. 2003 Nov 25;100(24):14409-14. doi: 10.1073/pnas.2332656100. Epub 2003 Nov 11. Proc Natl Acad Sci U S A. 2003. PMID: 14612566 Free PMC article.
-
A Flux Balance of Glucose Metabolism Clarifies the Requirements of the Warburg Effect.Biophys J. 2016 Sep 6;111(5):1088-100. doi: 10.1016/j.bpj.2016.07.028. Biophys J. 2016. PMID: 27602736 Free PMC article.
-
Decrease in oxidative phosphorylation yield in presence of butyrate in perfused liver isolated from fed rats.BMC Physiol. 2007 Aug 28;7:8. doi: 10.1186/1472-6793-7-8. BMC Physiol. 2007. PMID: 17725817 Free PMC article.
-
A metabolic link between mitochondrial ATP synthesis and liver glycogen metabolism: NMR study in rats re-fed with butyrate and/or glucose.Nutr Metab (Lond). 2011 Jun 15;8(1):38. doi: 10.1186/1743-7075-8-38. Nutr Metab (Lond). 2011. PMID: 21676253 Free PMC article.
-
Liver ATP synthesis is lower and relates to insulin sensitivity in patients with type 2 diabetes.Diabetes Care. 2011 Feb;34(2):448-53. doi: 10.2337/dc10-1076. Epub 2011 Jan 7. Diabetes Care. 2011. PMID: 21216854 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous
