Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues
- PMID: 28573167
- PMCID: PMC5448413
- DOI: 10.21769/BioProtoc.2037
Preparation of Purified Gram-positive Bacterial Cell Wall and Detection in Placenta and Fetal Tissues
Abstract
Cell wall is a complex biopolymer on the surface of all Gram-positive bacteria. During infection, cell wall is recognized by the innate immune receptor Toll-like receptor 2 causing intense inflammation and tissue damage. In animal models, cell wall traffics from the blood stream to many organs in the body, including brain, heart, placenta and fetus. This protocol describes how to prepare purified cell wall from Streptococcus pneumoniae, detect its distribution in animal tissues, and study the tissue response using the placenta and fetal brain as examples.
Keywords: Bacterial inflammation; Cell wall; Fetal neurogenesis; Neuroproliferation; Peptidoglycan; Placental trafficking; Streptococcus pneumonae; Toll like receptor 2 ligand.
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