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. 1988 Sep;62(9):3497-500.
doi: 10.1128/JVI.62.9.3497-3500.1988.

Epstein-Barr virus-positive and -negative B-cell lines can be infected with human immunodeficiency virus types 1 and 2

J E Monroe  1 , A Calender, C Mulder
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Epstein-Barr virus-positive and -negative B-cell lines can be infected with human immunodeficiency virus types 1 and 2

J E Monroe et al. J Virol. 1988 Sep.

Abstract

Human immunodeficiency virus type 1 (HIV-1) can infect CD4+ lymphocytes, monocytes-macrophages, and various other cell lines, including B-cell lines. To study the parameters of B-cell infections, we examined the susceptibility of 24 B-lymphoid cell lines to both HIV-1 and HIV-2 infections. These cell lines included a series of Epstein-Barr virus (EBV) genome-negative Burkitt's lymphoma cell lines and their EBV-converted counterparts. To infect these cells we used two HIV-1 isolates and one HIV-2 isolate. Infections were monitored with a cytoplasmic RNA dot-blot and a syncytium assay. HIV infection was also studied by a novel method based on electrophoresis of DNA liberated from cells that were lysed in situ in the well of an agarose gel. All human B-cell lines could be infected with HIV-1, regardless of the presence of EBV genomes; thus, EBV infection had no major effect on HIV susceptibility of B-cell lines. Integrated proviral HIV genomes could be detected by Southern blot analysis of DNA extracted from long-term, non-HIV-producing B-cell lines. This study suggests that B-lymphoid cells may serve as reservoirs for latent or persistent HIV infections in vivo, even in the absence of EBV infection.

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References

    1. Science. 1986 Nov 28;234(4780):1120-3 - PubMed
    1. Science. 1983 May 20;220(4599):868-71 - PubMed
    1. Nature. 1986 Dec 11-17;324(6097):572-5 - PubMed
    1. Nature. 1987 Jan 1-7;325(6099):67-70 - PubMed
    1. Proc Natl Acad Sci U S A. 1986 Dec;83(24):9759-63 - PubMed

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