Comprehensive metabonomic analysis of heart tissue from isoproterenol-induced myocardial infarction rat based on reversed-phase and hydrophilic interaction chromatography coupled to mass spectrometry
- PMID: 28371309
- DOI: 10.1002/jssc.201601013
Comprehensive metabonomic analysis of heart tissue from isoproterenol-induced myocardial infarction rat based on reversed-phase and hydrophilic interaction chromatography coupled to mass spectrometry
Abstract
We aim to describe the metabonomic characteristics of myocardial infarction rats. High-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was utilized to develop a metabonomic method of the heart homogenates of myocardial infarction rats. Hydrophilic interaction chromatography allows the analysis of high polar metabolites, providing complementary information to reversed-phase liquid chromatography. We combined reversed phase and hydrophilic interaction chromatographic separations to analyze 18 samples, ten from myocardial infarction rat hearts and eight from normal rat hearts. A total of 16 potential biomarkers in rat heart tissue were screened out, primarily related to oxidative stress, nitric oxide damage, taurine, and hypotaurine metabolism and sphingolipid metabolism. This research showed that a comprehensive metabonomic study is a useful tool to reveal the underlying mechanism of myocardial infarction.
Keywords: biomarkers; hydrophilic interaction chromatography; metabonomics; myocardial infarction; reversed-phase chromatography.
© 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Similar articles
-
Hydrophilic interaction and reversed-phase ultraperformance liquid chromatography TOF-MS for serum metabonomic analysis of myocardial infarction in rats and its applications.Mol Biosyst. 2012 Feb;8(2):548-56. doi: 10.1039/c1mb05324h. Epub 2011 Oct 31. Mol Biosyst. 2012. PMID: 22037674
-
Metabolic pathways involved in Xin-Ke-Shu protecting against myocardial infarction in rats using ultra high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry.J Pharm Biomed Anal. 2014 Mar;90:35-44. doi: 10.1016/j.jpba.2013.11.008. Epub 2013 Nov 15. J Pharm Biomed Anal. 2014. PMID: 24321516
-
The metabolic disturbances of isoproterenol induced myocardial infarction in rats based on a tissue targeted metabonomics.Mol Biosyst. 2013 Nov;9(11):2823-34. doi: 10.1039/c3mb70222g. Mol Biosyst. 2013. PMID: 24057015
-
Hydrophilic interaction chromatography coupled to MS for metabonomic/metabolomic studies.J Sep Sci. 2010 Mar;33(6-7):716-27. doi: 10.1002/jssc.200900803. J Sep Sci. 2010. PMID: 20187037 Review.
-
HILIC-MS for metabolomics: An attractive and complementary approach to RPLC-MS.Mass Spectrom Rev. 2016 Sep;35(5):574-600. doi: 10.1002/mas.21445. Epub 2014 Oct 3. Mass Spectrom Rev. 2016. PMID: 25284160 Review.
Cited by
-
Untargeted Metabolomics Studies of H9c2 Cardiac Cells Submitted to Oxidative Stress, β-Adrenergic Stimulation and Doxorubicin Treatment: Investigation of Cardiac Biomarkers.Front Mol Biosci. 2022 Jun 29;9:898742. doi: 10.3389/fmolb.2022.898742. eCollection 2022. Front Mol Biosci. 2022. PMID: 35847971 Free PMC article.
-
Sphingolipid Signature of Human Feto-Placental Vasculature in Preeclampsia.Int J Mol Sci. 2020 Feb 4;21(3):1019. doi: 10.3390/ijms21031019. Int J Mol Sci. 2020. PMID: 32033121 Free PMC article.
-
Modified Taohong Siwu decoction improves cardiac function after myocardial ischaemia and reperfusion in rats by promoting endogenous stem cell mobilization and regulating metabolites.Pharm Biol. 2022 Dec;60(1):1721-1731. doi: 10.1080/13880209.2022.2116054. Pharm Biol. 2022. PMID: 36086864 Free PMC article.
-
Getting to the heart of the sphingolipid riddle.Curr Opin Physiol. 2018 Feb;1:111-122. doi: 10.1016/j.cophys.2017.10.002. Epub 2017 Dec 13. Curr Opin Physiol. 2018. PMID: 33195889 Free PMC article.
-
Dihydrosphingosine driven enrichment of sphingolipids attenuates TGFβ induced collagen synthesis in cardiac fibroblasts.Int J Cardiol Heart Vasc. 2021 Jul 6;35:100837. doi: 10.1016/j.ijcha.2021.100837. eCollection 2021 Aug. Int J Cardiol Heart Vasc. 2021. PMID: 34277924 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical
