Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Mar;62(3):776-82.
doi: 10.1128/JVI.62.3.776-782.1988.

Expression of the M gene of vesicular stomatitis virus cloned in various vaccinia virus vectors

Y Li  1 , L Z Luo, R M Snyder, R R Wagner
Affiliations

Expression of the M gene of vesicular stomatitis virus cloned in various vaccinia virus vectors

Y Li et al. J Virol. 1988 Mar.

Abstract

Initial attempts to clone the matrix (M) gene of vesicular stomatitis virus (VSV) in a vaccinia virus expression vector failed, apparently because the expressed M protein, and particularly a carboxy-terminus-distal two-thirds fragment, was lethal for the virus recombinant. Therefore, a transient eucaryotic expression system was used in which a cDNA clone of the VSV M protein mRNA was inserted into a region of plasmid pTF7 flanked by the promoter and terminator sequences for the T7 bacteriophage RNA polymerase. When CV-1 cells infected with recombinant vaccinia virus vTF1-6,2 expressing the T7 RNA polymerase were transfected with pTF7-M3, the cells produced considerable amounts of M protein reactive by Western blot (immunoblot) analysis with monoclonal antibodies directed to VSV M protein. Evidence for biological activity of the plasmid-expressed wild-type M protein was provided by marker rescue of the M gene temperature-sensitive mutant tsO23(III) at the restrictive temperature. Somewhat higher levels of M protein expression were obtained in CV-1 cells coinfected with a vaccinia virus-M gene recombinant under control of the T7 polymerase promoter along with T7 polymerase-expressing vaccinia virus vTF1-6,2.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. J Virol. 1975 Jul;16(1):75-84 - PubMed
    1. Mol Cell Biol. 1987 Jul;7(7):2538-44 - PubMed
    1. Biochemistry. 1976 Aug 10;15(16):3563-70 - PubMed
    1. J Virol. 1977 Mar;21(3):1140-8 - PubMed
    1. J Virol. 1977 Mar;21(3):1149-58 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources