Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer
- PMID: 2827377
- DOI: 10.1016/0042-6822(88)90408-4
Evidence that the mature form of the flavivirus nonstructural protein NS1 is a dimer
Abstract
Evidence is presented which indicates that the dengue-2 virus nonstructural protein NS1 (soluble complement fixing antigen) exists in infected BHK and mosquito cell cultures as part of a stable oligomer. Identification of the dissociation products of the isolated oligomer and comparison of the number of N-linked glycans in native and denatured NS1 is consistent with the idea that the high-molecular-weight form of NS1 is a homodimer. By analyzing lysates of BHK cells infected with St. Louis encephalitis virus or Powassan virus and proteins from dengue-2 virus-infected mouse brain we have demonstrated that the appearance of the high-molecular-weight form of NS1 is a general feature of flavivirus infection. It is formed between 20 and 40 min after NS1 is synthesized and before the protein passes the Golgi apparatus. Both soluble and pelletable extracellular NS1 are also found as the high-molecular-weight form.
Similar articles
-
Newly synthesized dengue-2 virus nonstructural protein NS1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization.Virology. 1989 Jul;171(1):302-5. doi: 10.1016/0042-6822(89)90544-8. Virology. 1989. PMID: 2525840
-
Cleavage of dengue virus NS1-NS2A requires an octapeptide sequence at the C terminus of NS1.J Virol. 1990 Sep;64(9):4573-7. doi: 10.1128/JVI.64.9.4573-4577.1990. J Virol. 1990. PMID: 2143546 Free PMC article.
-
Production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein NS1.J Gen Virol. 1991 Apr;72 ( Pt 4):961-5. doi: 10.1099/0022-1317-72-4-961. J Gen Virol. 1991. PMID: 1849979
-
Secretion of Nonstructural Protein 1 of Dengue Virus from Infected Mosquito Cells: Facts and Speculations.J Virol. 2018 Jun 29;92(14):e00275-18. doi: 10.1128/JVI.00275-18. Print 2018 Jul 15. J Virol. 2018. PMID: 29720514 Free PMC article. Review.
-
The flavivirus NS1 protein: molecular and structural biology, immunology, role in pathogenesis and application as a diagnostic biomarker.Antiviral Res. 2013 May;98(2):192-208. doi: 10.1016/j.antiviral.2013.03.008. Epub 2013 Mar 21. Antiviral Res. 2013. PMID: 23523765 Review.
Cited by
-
Flavivirus NS1: a multifaceted enigmatic viral protein.Virol J. 2016 Jul 29;13:131. doi: 10.1186/s12985-016-0590-7. Virol J. 2016. PMID: 27473856 Free PMC article. Review.
-
Maturation of Japanese encephalitis virus glycoproteins produced by infected mammalian and mosquito cells.Virology. 1989 Apr;169(2):354-64. doi: 10.1016/0042-6822(89)90161-x. Virology. 1989. PMID: 2523178 Free PMC article.
-
Development of a quantitative NS1 antigen enzyme-linked immunosorbent assay (ELISA) for Zika virus detection using a novel virus-specific mAb.Sci Rep. 2024 Jan 30;14(1):2544. doi: 10.1038/s41598-024-52123-2. Sci Rep. 2024. PMID: 38291109 Free PMC article.
-
Characterization of a siberian virus isolated from a patient with progressive chronic tick-borne encephalitis.J Virol. 2003 Jan;77(1):25-36. doi: 10.1128/jvi.77.1.25-36.2003. J Virol. 2003. PMID: 12477807 Free PMC article.
-
Defining the levels of secreted non-structural protein NS1 after West Nile virus infection in cell culture and mice.J Med Virol. 2008 Mar;80(3):547-56. doi: 10.1002/jmv.21091. J Med Virol. 2008. PMID: 18205232 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Other Literature Sources