Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Sep;209(2):366-73.
doi: 10.1007/BF00329667.

Isolation and expression analysis of two yeast regulatory genes involved in the derepression of glucose-repressible enzymes

H J Schüller  1 , K D Entian
Affiliations

Isolation and expression analysis of two yeast regulatory genes involved in the derepression of glucose-repressible enzymes

H J Schüller et al. Mol Gen Genet. 1987 Sep.

Abstract

Yeast strains carrying one of the two regulatory mutations cat1 and cat3 are defective in derepression of several glucose-repressible enzymes that are necessary for utilizing non-fermentable carbon sources. Hence, these strains fail to grow on ethanol, glycerol or acetate. The synthesis of isocitrate lyase, malate synthase, malate dehydrogenase and fructose-1,6-bisphosphatase is strongly affected in cat1 and cat3 strains. Genes CAT1 and CAT3 have been isolated by complementation of the cognate mutations after transformation with an episomal plasmid gene library. The restriction map of CAT1 proved its allelism to the earlier isolated SNF1 gene. Both genes appear to exist as single-copy genes per haploid genome as indicated by Southern hybridization. Northern analysis has shown that the 1.35 kb CAT3 mRNA is constitutively expressed, independent of the carbon source in the medium. Derepression studies with CAT3 transformants using a multi-copy plasmid showed over-expression of glyoxylate cycle enzymes. This result would be consistent with a direct effector function for the CAT3 gene product.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2119-23 - PubMed
    1. Mol Cell Biol. 1984 Aug;4(8):1440-8 - PubMed
    1. Mol Cell Biol. 1986 Jul;6(7):2324-33 - PubMed
    1. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5374-8 - PubMed
    1. Mol Gen Genet. 1977 Feb 28;151(1):95-103 - PubMed

Publication types

LinkOut - more resources